RRC ID 33952
著者 Cortés JC, Pujol N, Sato M, Pinar M, Ramos M, Moreno B, Osumi M, Ribas JC, Pérez P.
タイトル Cooperation between Paxillin-like Protein Pxl1 and Glucan Synthase Bgs1 Is Essential for Actomyosin Ring Stability and Septum Formation in Fission Yeast.
ジャーナル PLoS Genet
Abstract In fungal cells cytokinesis requires coordinated closure of a contractile actomyosin ring (CAR) and synthesis of a special cell wall structure known as the division septum. Many CAR proteins have been identified and characterized, but how these molecules interact with the septum synthesis enzymes to form the septum remains unclear. Our genetic study using fission yeast shows that cooperation between the paxillin homolog Pxl1, required for ring integrity, and Bgs1, the enzyme responsible for linear β(1,3)glucan synthesis and primary septum formation, is required for stable anchorage of the CAR to the plasma membrane before septation onset, and for cleavage furrow formation. Thus, lack of Pxl1 in combination with Bgs1 depletion, causes failure of ring contraction and lateral cell wall overgrowth towards the cell lumen without septum formation. We also describe here that Pxl1 concentration at the CAR increases during cytokinesis and that this increase depends on the SH3 domain of the F-BAR protein Cdc15. In consequence, Bgs1 depletion in cells carrying a cdc15ΔSH3 allele causes ring disassembly and septation blockage, as it does in cells lacking Pxl1. On the other hand, the absence of Pxl1 is lethal when Cdc15 function is affected, generating a large sliding of the CAR with deposition of septum wall material along the cell cortex, and suggesting additional functions for both Pxl1 and Cdc15 proteins. In conclusion, our findings indicate that CAR anchorage to the plasma membrane through Cdc15 and Pxl1, and concomitant Bgs1 activity, are necessary for CAR maintenance and septum formation in fission yeast.
巻・号 11(7)
ページ e1005358
公開日 2015-7-1
DOI 10.1371/journal.pgen.1005358
PII PGENETICS-D-14-03114
PMID 26132084
PMC PMC4489101
MeSH Actin Cytoskeleton / metabolism Actomyosin / chemistry Actomyosin / metabolism* Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Cell Membrane / metabolism Cell Surface Extensions / metabolism* Cell Wall / metabolism Cytokinesis / genetics Cytokinesis / physiology Cytoskeletal Proteins / genetics Cytoskeletal Proteins / metabolism* GTP-Binding Proteins / genetics GTP-Binding Proteins / metabolism Glucosyltransferases / genetics Glucosyltransferases / metabolism* Membrane Proteins / genetics Membrane Proteins / metabolism Paxillin / metabolism Protein Structure, Tertiary Schizosaccharomyces / genetics* Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* beta-Glucans / metabolism
IF 5.175
引用数 22
WOS 分野 GENETICS & HEREDITY
リソース情報
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