RRC ID 38126
著者 Mizutani T, Li R, Haga H, Kawabata K.
タイトル Transgene integration into the human AAVS1 locus enhances myosin II-dependent contractile force by reducing expression of myosin binding subunit 85.
ジャーナル Biochem Biophys Res Commun
Abstract The adeno-associated virus site 1 (AAVS1) locus in the human genome is a strong candidate for gene therapy by insertion of an exogenous gene into the locus. The AAVS1 locus includes the coding region for myosin binding subunit 85 (MBS85). Although the function of MBS85 is not well understood, myosin II-dependent contractile force may be affected by altered expression of MBS85. The effect of altered expression of MBS85 on cellular contractile force should be examined prior to the application of gene therapy. In this study, we show that transgene integration into AAVS1 and consequent reduction of MBS85 expression changes myosin II-dependent cellular contractile force. We established a human fibroblast cell line with exogenous DNA knocked-in to AAVS1 (KI cells) using the CRISPR/Cas9 genome editing system. Western blotting analysis showed that KI cells had significantly reduced MBS85 expression. KI cells also showed greater cellular contractile force than control cells. The increased contractile force was associated with phosphorylation of the myosin II regulatory light chain (MRLC). Transfection of KI cells with an MBS85 expression plasmid restored cellular contractile force and phosphorylation of MRLC to the levels in control cells. These data suggest that transgene integration into the human AAVS1 locus induces an increase in cellular contractile force and thus should be considered as a gene therapy to effect changes in cellular contractile force.
巻・号 465(2)
ページ 270-4
公開日 2015-9-18
DOI 10.1016/j.bbrc.2015.08.018
PII S0006-291X(15)30408-3
PMID 26260320
MeSH Bacterial Proteins / genetics Bacterial Proteins / metabolism CRISPR-Associated Protein 9 Cell Engineering Cell Line Clustered Regularly Interspaced Short Palindromic Repeats Dependovirus / genetics Dependovirus / metabolism Endonucleases / genetics Endonucleases / metabolism Fibroblasts / cytology Fibroblasts / metabolism* Gene Knock-In Techniques Genes, Reporter Genetic Loci* Genome, Human* Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Humans Muscle Contraction / genetics Myosin Light Chains / genetics Myosin Light Chains / metabolism* Myosin Type II / genetics Myosin Type II / metabolism* Plasmids / chemistry Plasmids / metabolism Protein Phosphatase 1 / genetics Protein Phosphatase 1 / metabolism* Transgenes
IF 2.985
引用数 8
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 MRC-5 SV1 TG1(RCB0207)