RRC ID 45415
著者 Fujioka K, Kishida T, Ejima A, Yamamoto K, Fujii W, Murakami K, Seno T, Yamamoto A, Kohno M, Oda R, Yamamoto T, Fujiwara H, Kawahito Y, Mazda O.
タイトル Inhibition of osteoclastogenesis by osteoblast-like cells genetically engineered to produce interleukin-10.
ジャーナル Biochem Biophys Res Commun
Abstract Bone destruction at inflamed joints is an important complication associated with rheumatoid arthritis (RA). Interleukin-10 (IL-10) may suppress not only inflammation but also induction of osteoclasts that play key roles in the bone destruction. If IL-10-producing osteoblast-like cells are induced from patient somatic cells and transplanted back into the destructive bone lesion, such therapy may promote bone remodeling by the cooperative effects of IL-10 and osteoblasts. We transduced mouse fibroblasts with genes for IL-10 and Runx2 that is a crucial transcription factor for osteoblast differentiation. The IL-10-producing induced osteoblast-like cells (IL-10-iOBs) strongly expressed osteoblast-specific genes and massively produced bone matrix that were mineralized by calcium phosphate in vitro and in vivo. Culture supernatant of IL-10-iOBs significantly suppressed induction of osteoclast from RANKL-stimulated Raw264.7 cells as well as LPS-induced production of inflammatory cytokine by macrophages. The IL-10-iOBs may be applicable to novel cell-based therapy against bone destruction associated with RA.
巻・号 456(3)
ページ 785-91
公開日 2015-1-16
DOI 10.1016/j.bbrc.2014.12.040
PII S0006-291X(14)02205-0
PMID 25514036
MeSH Animals Arthritis, Rheumatoid / complications Bone Matrix / immunology Bone Remodeling Bone Resorption / etiology Bone Resorption / therapy* Calcification, Physiologic Calcium Phosphates / metabolism Cell Differentiation Cell Line Cell- and Tissue-Based Therapy / methods* Core Binding Factor Alpha 1 Subunit / genetics Core Binding Factor Alpha 1 Subunit / immunology Gene Expression Regulation Genetic Engineering Interleukin-10 / genetics Interleukin-10 / immunology* Lipopolysaccharides / immunology Macrophages / immunology Mice Osteoblasts / immunology* Osteoclasts / immunology* Osteogenesis / genetics RANK Ligand / immunology Transduction, Genetic
IF 2.985
引用数 6
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 RAW 264(RCB0535)