RRC ID 34887
著者 Dequéant ML, Fagegaltier D, Hu Y, Spirohn K, Simcox A, Hannon GJ, Perrimon N.
タイトル Discovery of progenitor cell signatures by time-series synexpression analysis during Drosophila embryonic cell immortalization.
ジャーナル Proc Natl Acad Sci U S A
Abstract The use of time series profiling to identify groups of functionally related genes (synexpression groups) is a powerful approach for the discovery of gene function. Here we apply this strategy during Ras(V12) immortalization of Drosophila embryonic cells, a phenomenon not well characterized. Using high-resolution transcriptional time-series datasets, we generated a gene network based on temporal expression profile similarities. This analysis revealed that common immortalized cells are related to adult muscle precursors (AMPs), a stem cell-like population contributing to adult muscles and sharing properties with vertebrate satellite cells. Remarkably, the immortalized cells retained the capacity for myogenic differentiation when treated with the steroid hormone ecdysone. Further, we validated in vivo the transcription factor CG9650, the ortholog of mammalian Bcl11a/b, as a regulator of AMP proliferation predicted by our analysis. Our study demonstrates the power of time series synexpression analysis to characterize Drosophila embryonic progenitor lines and identify stem/progenitor cell regulators.
巻・号 112(42)
ページ 12974-9
公開日 2015-10-20
DOI 10.1073/pnas.1517729112
PII 1517729112
PMID 26438832
PMC PMC4620889
MeSH Animals Cell Differentiation Cell Line, Transformed* Cell Proliferation Drosophila / embryology* Embryo, Nonmammalian / cytology Promoter Regions, Genetic Retinoblastoma Protein / metabolism Stem Cells / cytology* Transcription, Genetic
IF 9.412
引用数 14
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
ショウジョウバエ 9650R-1 9650R-4