RRC ID 47428
著者 Yang L, Paul S, Trieu KG, Dent LG, Froldi F, Forés M, Webster K, Siegfried KR, Kondo S, Harvey K, Cheng L, Jiménez G, Shvartsman SY, Veraksa A.
タイトル Minibrain and Wings apart control organ growth and tissue patterning through down-regulation of Capicua.
ジャーナル Proc Natl Acad Sci U S A
Abstract The transcriptional repressor Capicua (Cic) controls tissue patterning and restricts organ growth, and has been recently implicated in several cancers. Cic has emerged as a primary sensor of signaling downstream of the receptor tyrosine kinase (RTK)/extracellular signal-regulated kinase (ERK) pathway, but how Cic activity is regulated in different cellular contexts remains poorly understood. We found that the kinase Minibrain (Mnb, ortholog of mammalian DYRK1A), acting through the adaptor protein Wings apart (Wap), physically interacts with and phosphorylates the Cic protein. Mnb and Wap inhibit Cic function by limiting its transcriptional repressor activity. Down-regulation of Cic by Mnb/Wap is necessary for promoting the growth of multiple organs, including the wings, eyes, and the brain, and for proper tissue patterning in the wing. We have thus uncovered a previously unknown mechanism of down-regulation of Cic activity by Mnb and Wap, which operates independently from the ERK-mediated control of Cic. Therefore, Cic functions as an integrator of upstream signals that are essential for tissue patterning and organ growth. Finally, because DYRK1A and CIC exhibit, respectively, prooncogenic vs. tumor suppressor activities in human oligodendroglioma, our results raise the possibility that DYRK1A may also down-regulate CIC in human cells.
巻・号 113(38)
ページ 10583-8
公開日 2016-9-20
DOI 10.1073/pnas.1609417113
PII 1609417113
PMID 27601662
PMC PMC5035877
MeSH Adaptor Proteins, Signal Transducing / genetics Animals Body Patterning / genetics* Drosophila / genetics* Drosophila / growth & development Drosophila Proteins / biosynthesis Drosophila Proteins / genetics* Gene Expression Regulation, Developmental HMGB Proteins / biosynthesis HMGB Proteins / genetics* Humans Neoplasms / genetics Phosphorylation Protein Serine-Threonine Kinases / biosynthesis Protein Serine-Threonine Kinases / genetics* Protein-Tyrosine Kinases / genetics* Repressor Proteins / biosynthesis Repressor Proteins / genetics* Wings, Animal / growth & development
IF 9.412
引用数 17
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
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