RRC ID 47711
著者 Thankachan JM, Nuthalapati SS, Addanki Tirumala N, Ananthanarayanan V.
タイトル Fission yeast myosin I facilitates PI(4,5)P2-mediated anchoring of cytoplasmic dynein to the cortex.
ジャーナル Proc Natl Acad Sci U S A
Abstract Several key processes in the cell, such as vesicle transport and spindle positioning, are mediated by the motor protein cytoplasmic dynein, which produces force on the microtubule. For the functions that require movement of the centrosome and the associated nuclear material, dynein needs to have a stable attachment at the cell cortex. In fission yeast, Mcp5 is the anchor protein of dynein and is required for the oscillations of the horsetail nucleus during meiotic prophase. Although the role of Mcp5 in anchoring dynein to the cortex has been identified, it is unknown how Mcp5 associates with the membrane as well as the importance of the underlying attachment to the nuclear oscillations. Here, we set out to quantify Mcp5 organization and identify the binding partner of Mcp5 at the membrane. We used confocal and total internal reflection fluorescence microscopy to count the number of Mcp5 foci and the number of Mcp5 molecules in an individual focus. Further, we quantified the localization pattern of Mcp5 in fission yeast zygotes and show by perturbation of phosphatidylinositol 4-phosphate 5-kinase that Mcp5 binds to phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2]. Remarkably, we discovered that the myosin I protein in fission yeast, Myo1, which is required for organization of sterol-rich domains in the cell membrane, facilitates the localization of Mcp5 and that of cytoplasmic dynein on the membrane. Finally, we demonstrate that Myo1-facilitated association of Mcp5 and dynein to the membrane determines the dynamics of nuclear oscillations and, in essence, dynein activity.
巻・号 114(13)
ページ E2672-E2681
公開日 2017-3-28
DOI 10.1073/pnas.1615883114
PII 1615883114
PMID 28292899
PMC PMC5380020
MeSH Binding Sites Cytoplasm / metabolism Cytoplasmic Dyneins / metabolism* Fungal Proteins / analysis Fungal Proteins / chemistry Fungal Proteins / physiology* Myosin Type I / analysis Myosin Type I / chemistry Myosin Type I / physiology* Schizosaccharomyces
IF 9.412
引用数 6
WOS 分野 CELL BIOLOGY
リソース情報
酵母 FY12794 FY13570 FY13572 FY13579 FY15622 FY16831 FY16854 FY16855 FY16951 FYP2932 FYP2934