RRC ID 49228
著者 Gao C, Langbein L, Kamal F, George AA, Walworth NC.
タイトル Kinetochore Components Required for Centromeric Chromatin Assembly Are Impacted by Msc1 in Schizosaccharomyces pombe.
ジャーナル Genetics
Abstract Eukaryotic chromosome segregation requires a protein complex known as the kinetochore that mediates attachment between mitotic spindle microtubules and centromere-specific nucleosomes composed of the widely conserved histone variant CENP-A. Mutations in kinetochore proteins of the fission yeast Schizosaccharomyces pombe lead to chromosome missegregation such that daughter cells emerge from mitosis with unequal DNA content. We find that multiple copies of Msc1-a fission yeast homolog of the KDM5 family of proteins-suppresses the temperature-sensitive growth defect of several kinetochore mutants, including mis16 and mis18, as well as mis6, mis15, and mis17, components of the Constitutive Centromere Associated Network (CCAN). On the other hand, deletion of msc1 exacerbates both the growth defect and chromosome missegregation phenotype of each of these mutants. The C-terminal PHD domains of Msc1, previously shown to associate with a histone deacetylase activity, are necessary for Msc1 function when kinetochore mutants are compromised. We also demonstrate that, in the absence of Msc1, the frequency of localization to the kinetochore of Mis16 and Mis15 is altered from wild-type cells. As we show here for msc1, others have shown that elevating cnp1 levels acts similarly to promote survival of the CCAN mutants. The rescue of mis15 and mis17 by cnp1 is, however, independent of msc1 Thus, Msc1 appears to contribute to the chromatin environment at the centromere: the absence of Msc1 sensitizes cells to perturbations in kinetochore function, while elevating Msc1 overcomes loss of function of critical components of the kinetochore and centromere.
巻・号 207(2)
ページ 559-569
公開日 2017-10-1
DOI 10.1534/genetics.117.300183
PII genetics.117.300183
PMID 28827290
PMC PMC5629323
MeSH Carrier Proteins / genetics Carrier Proteins / metabolism Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Centromere / genetics Centromere / metabolism Chromatin / genetics* Chromatin / metabolism Chromatin Assembly and Disassembly Chromosomal Proteins, Non-Histone / genetics Chromosomal Proteins, Non-Histone / metabolism Chromosome Segregation DNA-Binding Proteins / chemistry DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Kinetochores / metabolism* Protein Domains Schizosaccharomyces / genetics* Schizosaccharomyces pombe Proteins / chemistry Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism*
IF 4.015
引用数 0
リソース情報
酵母 FY20041 FY20042 FY20043 FY20044 FY10474 FY10468