RRC ID 37432
著者 Zhang Z, Wang B, Hu Q, Sommerfeld M, Li Y, Han D.
タイトル A new paradigm for producing astaxanthin from the unicellular green alga Haematococcus pluvialis.
ジャーナル Biotechnol Bioeng
Abstract The unicellular green alga Haematococcus pluvialis has been exploited as a cell factory to produce the high-value antioxidant astaxanthin for over two decades, due to its superior ability to synthesize astaxanthin under adverse culture conditions. However, slow vegetative growth under favorable culture conditions and cell deterioration or death under stress conditions (e.g., high light, nitrogen starvation) has limited the astaxanthin production. In this study, a new paradigm that integrated heterotrophic cultivation, acclimation of heterotrophically grown cells to specific light/nutrient regimes, followed by induction of astaxanthin accumulation under photoautotrophic conditions was developed. First, the environmental conditions such as pH, carbon source, nitrogen regime, and light intensity, were optimized to induce astaxanthin accumulation in the dark-grown cells. Although moderate astaxanthin content (e.g., 1% of dry weight) and astaxanthin productivity (2.5 mg L(-1)  day(-1) ) were obtained under the optimized conditions, a considerable number of cells died off when subjected to stress for astaxanthin induction. To minimize the susceptibility of dark-grown cells to light stress, the algal cells were acclimated, prior to light induction of astaxanthin biosynthesis, under moderate illumination in the presence of nitrogen. Introduction of this strategy significantly reduced the cell mortality rate under high-light and resulted in increased cellular astaxanthin content and astaxanthin productivity. The productivity of astaxanthin was further improved to 10.5 mg L(-1)  day(-1) by implementation of such a strategy in a bubbling column photobioreactor. Biochemical and physiological analyses suggested that rebuilding of photosynthetic apparatus including D1 protein and PsbO, and recovery of PSII activities, are essential for acclimation of dark-grown cells under photo-induction conditions. Biotechnol. Bioeng. 2016;113: 2088-2099. © 2016 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc.
巻・号 113(10)
ページ 2088-99
公開日 2016-10-1
DOI 10.1002/bit.25976
PMID 27563850
PMC PMC5071682
MeSH Cell Culture Techniques / methods Chlorophyta / metabolism* Dose-Response Relationship, Radiation Light Nitrogen / metabolism* Photic Stimulation / methods Photobioreactors / microbiology* Photosynthesis / physiology* Photosynthesis / radiation effects Radiation Dosage Xanthophylls / biosynthesis Xanthophylls / isolation & purification Xanthophylls / radiation effects
IF 4.002
引用数 24
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
リソース情報
藻類 NIES-144