RRC ID 37461
著者 Kobayashi A, Sekiguchi Y, Oroguchi T, Okajima K, Fukuda A, Oide M, Yamamoto M, Nakasako M.
タイトル Specimen preparation for cryogenic coherent X-ray diffraction imaging of biological cells and cellular organelles by using the X-ray free-electron laser at SACLA.
ジャーナル J Synchrotron Radiat
Abstract Coherent X-ray diffraction imaging (CXDI) allows internal structures of biological cells and cellular organelles to be analyzed. CXDI experiments have been conducted at 66 K for frozen-hydrated biological specimens at the SPring-8 Angstrom Compact Free-Electron Laser facility (SACLA). In these cryogenic CXDI experiments using X-ray free-electron laser (XFEL) pulses, specimen particles dispersed on thin membranes of specimen disks are transferred into the vacuum chamber of a diffraction apparatus. Because focused single XFEL pulses destroy specimen particles at the atomic level, diffraction patterns are collected through raster scanning the specimen disks to provide fresh specimen particles in the irradiation area. The efficiency of diffraction data collection in cryogenic experiments depends on the quality of the prepared specimens. Here, detailed procedures for preparing frozen-hydrated biological specimens, particularly thin membranes and devices developed in our laboratory, are reported. In addition, the quality of the frozen-hydrated specimens are evaluated by analyzing the characteristics of the collected diffraction patterns. Based on the experimental results, the internal structures of the frozen-hydrated specimens and the future development for efficient diffraction data collection are discussed.
巻・号 23(Pt 4)
ページ 975-89
公開日 2016-7-1
DOI 10.1107/S1600577516007736
PII S1600577516007736
PMID 27359147
PMC PMC5357008
MeSH Electrons Lasers Organelles X-Ray Diffraction* X-Rays
IF 2.251
引用数 8
WOS 分野 INSTRUMENTS & INSTRUMENTATION OPTICS PHYSICS, APPLIED
リソース情報
藻類 NIES-2087