論文 - 詳細
| RRC ID | 47212 |
|---|---|
| 著者 | Chiu YT, Yen HK, Lin TF. |
| タイトル | An alternative method to quantify 2-MIB producing cyanobacteria in drinking water reservoirs: Method development and field applications. |
| ジャーナル | Environ Res |
| Abstract |
2-Methylisoborneol (2-MIB) is a commonly detected cyanobacterial odorant in drinking water sources in many countries. To provide safe and high-quality water, development of a monitoring method for the detection of 2-MIB-synthesis (mibC) genes is very important. In this study, new primers MIBS02F/R intended specifically for the mibC gene were developed and tested. Experimental results show that the MIBS02F/R primer set was able to capture 13 2-MIB producing cyanobacterial strains grown in the laboratory, and to effectively amplify the targeted DNA region from 17 2-MIB-producing cyanobacterial strains listed in the literature. The primers were further coupled with a TaqMan probe to detect 2-MIB producers in 29 drinking water reservoirs (DWRs). The results showed statistically significant correlations between mibC genes and 2-MIB concentrations for the data from each reservoir (R2=0.413-0.998; p<0.05), from all reservoirs in each of the three islands (R2=0.302-0.796; p<0.01), and from all data of the three islands (R2=0.473-0.479; p<0.01). The results demonstrate that the real-time PCR can be an alternative method to provide information to managers of reservoirs and water utilities facing 2-MIB-related incidents. |
| 巻・号 | 151 |
| ページ | 618-627 |
| 公開日 | 2016-11-1 |
| DOI | 10.1016/j.envres.2016.08.034 |
| PII | S0013-9351(16)30505-9 |
| PMID | 27607443 |
| MeSH | Camphanes / analysis* Camphanes / metabolism Cyanobacteria / genetics Cyanobacteria / growth & development* Cyanobacteria / metabolism Drinking Water* / chemistry Drinking Water* / microbiology Genes, Bacterial* Real-Time Polymerase Chain Reaction Water Microbiology / standards* Water Resources / supply & distribution* |
| IF | 5.715 |
| 引用数 | 10 |
| WOS 分野 | PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH ENVIRONMENTAL SCIENCES |
| リソース情報 | |
| 藻類 | NIES-81 NIES-824 NIES-2104 NIES-2116 NIES-905 NIES-512 |