RRC ID 48517
著者 Higuchi-Takeuchi M, Morisaki K, Toyooka K, Numata K.
タイトル Synthesis of High-Molecular-Weight Polyhydroxyalkanoates by Marine Photosynthetic Purple Bacteria.
ジャーナル PLoS One
Abstract Polyhydroxyalkanoate (PHA) is a biopolyester/bioplastic that is produced by a variety of microorganisms to store carbon and increase reducing redox potential. Photosynthetic bacteria convert carbon dioxide into organic compounds using light energy and are known to accumulate PHA. We analyzed PHAs synthesized by 3 purple sulfur bacteria and 9 purple non-sulfur bacteria strains. These 12 purple bacteria were cultured in nitrogen-limited medium containing acetate and/or sodium bicarbonate as carbon sources. PHA production in the purple sulfur bacteria was induced by nitrogen-limited conditions. Purple non-sulfur bacteria accumulated PHA even under normal growth conditions, and PHA production in 3 strains was enhanced by nitrogen-limited conditions. Gel permeation chromatography analysis revealed that 5 photosynthetic purple bacteria synthesized high-molecular-weight PHAs, which are useful for industrial applications. Quantitative reverse transcription polymerase chain reaction analysis revealed that mRNA levels of phaC and PhaZ genes were low under nitrogen-limited conditions, resulting in production of high-molecular-weight PHAs. We conclude that all 12 tested strains are able to synthesize PHA to some degree, and we identify 5 photosynthetic purple bacteria that accumulate high-molecular-weight PHA molecules. Furthermore, the photosynthetic purple bacteria synthesized PHA when they were cultured in seawater supplemented with acetate. The photosynthetic purple bacteria strains characterized in this study should be useful as host microorganisms for large-scale PHA production utilizing abundant marine resources and carbon dioxide.
巻・号 11(8)
ページ e0160981
公開日 2016-1-1
DOI 10.1371/journal.pone.0160981
PII PONE-D-16-26334
PMID 27513570
PMC PMC4981452
MeSH Bacterial Proteins / metabolism* Molecular Weight Photosynthesis* Polyhydroxyalkanoates / biosynthesis* Proteobacteria / metabolism*
IF 2.74
引用数 27
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
リソース情報
一般微生物 JCM 14796 JCM 14262 JCM 17835 JCM 13911 JCM 13533 JCM 14780 JCM 14889 JCM 14149 JCM 14150 JCM 14544 JCM 13532 JCM 9337 JCM 13300 JCM 13589 JCM 13531