RRC ID 50064
著者 Davis TL, Rebay I.
タイトル Antagonistic regulation of the second mitotic wave by Eyes absent-Sine oculis and Combgap coordinates proliferation and specification in the Drosophila retina.
ジャーナル Development
Abstract The transition from proliferation to specification is fundamental to the development of appropriately patterned tissues. In the developing Drosophila eye, Eyes absent (Eya) and Sine oculis (So) orchestrate the progression of progenitor cells from asynchronous cell division to G1 arrest and neuronal specification at the morphogenetic furrow. Here, we uncover a novel role for Eya and So in promoting cell cycle exit in the second mitotic wave (SMW), a synchronized, terminal cell division that occurs several hours after passage of the furrow. We show that Combgap (Cg), a zinc-finger transcription factor, antagonizes Eya-So function in the SMW. Based on the ability of Cg to attenuate Eya-So transcriptional output in vivo and in cultured cells and on meta analysis of their chromatin occupancy profiles, we speculate that Cg limits Eya-So activation of select target genes posterior to the furrow to ensure properly timed mitotic exit. Our work supports a model in which context-specific modulation of transcriptional activity enables Eya and So to promote both entry into and exit from the cell cycle in a distinct spatiotemporal sequence.
巻・号 144(14)
ページ 2640-2651
公開日 2017-7-15
DOI 10.1242/dev.147231
PII dev.147231
PMID 28619818
PMC PMC5536924
MeSH Animals Animals, Genetically Modified Cell Cycle Cell Lineage Cell Proliferation Cell Survival Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / genetics Drosophila melanogaster / growth & development* Drosophila melanogaster / metabolism* Eye Proteins / genetics Eye Proteins / metabolism* Gene Expression Regulation, Developmental Gene Regulatory Networks Genes, Insect Homeodomain Proteins / genetics Homeodomain Proteins / metabolism* Mitosis Mutation Retina / cytology Retina / growth & development* Retina / metabolism* Transcription Factors / genetics Transcription Factors / metabolism*
IF 5.611
引用数 3
リソース情報
ショウジョウバエ 1395R-1 1395R-2