RRC ID 41058
著者 Techaruvichit P, Takahashi H, Vesaratchavest M, Keeratipibul S, Kuda T, Kimura B.
タイトル Development of Multiple-Locus Variable-Number Tandem-Repeat Analysis for Molecular Subtyping of Campylobacter jejuni by Using Capillary Electrophoresis.
ジャーナル Appl Environ Microbiol
Abstract Campylobacter jejuni is a common cause of the frequently reported food-borne diseases in developed and developing nations. This study describes the development of multiple-locus variable-number tandem-repeat (VNTR) analysis (MLVA) using capillary electrophoresis as a novel typing method for microbial source tracking and epidemiological investigation of C. jejuni. Among 36 tandem repeat loci detected by the Tandem Repeat Finder program, 7 VNTR loci were selected and used for characterizing 60 isolates recovered from chicken meat samples from retail shops, samples from chicken meat processing factory, and stool samples. The discrimination ability of MLVA was compared with that of multilocus sequence typing (MLST). MLVA (diversity index of 0.97 with 31 MLVA types) provided slightly higher discrimination than MLST (diversity index of 0.95 with 25 MLST types). The overall concordance between MLVA and MLST was estimated at 63% by adjusted Rand coefficient. MLVA predicted MLST type better than MLST predicted MLVA type, as reflected by Wallace coefficient (Wallace coefficient for MLVA to MLST versus MLST to MLVA, 86% versus 51%). MLVA is a useful tool and can be used for effective monitoring of C. jejuni and investigation of epidemics caused by C. jejuni.
巻・号 81(16)
ページ 5318-25
公開日 2015-8-15
DOI 10.1128/AEM.01151-15
PII AEM.01151-15
PMID 26025899
PMC PMC4510157
MeSH Animals Campylobacter jejuni / classification* Campylobacter jejuni / genetics* Campylobacter jejuni / isolation & purification Chickens DNA, Bacterial / chemistry DNA, Bacterial / genetics* Electrophoresis, Capillary / methods* Environmental Microbiology Feces / microbiology Food Handling Meat / microbiology Minisatellite Repeats* Molecular Epidemiology / methods Molecular Sequence Data Molecular Typing / methods* Sequence Analysis, DNA
IF 4.016
引用数 4
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
リソース情報
一般微生物 JCM 2013