RRC ID 52410
著者 Matsumoto K, Shimo T, Kurio N, Okui T, Ibaragi S, Kunisada Y, Obata K, Masui M, Pai P, Horikiri Y, Yamanaka N, Takigawa M, Sasaki A.
タイトル Low-intensity pulsed ultrasound stimulation promotes osteoblast differentiation through hedgehog signaling.
ジャーナル J Cell Biochem
Abstract Low-intensity pulsed ultrasound (LIPUS) has been used as an adjunct to fracture healing therapies, but the mechanisms underlying its action are not known. We reported that sonic hedgehog (SHH) signaling was activated in osteoblasts at the dynamic remodeling site of a bone fracture. Mechanical stimulation is a crucial factor in bone remodeling, and it is related to the primary cilia as a sensor of hedgehog signaling. Here we observed that LIPUS promoted callus formation in accord with Gli2-positive cells after 14 days at the mouse femur fractured site compared with a control group. An immunofluorescence analysis showed that the numbers of primary cilia and cilia/osterix double-positive osteoblasts were increased at the fracture site by LIPUS. LIPUS stimulated not only the number and the length of primary cilia, but also the levels of ciliated protein, Ift88 mRNA, and SHH, Gli1, and Gli2 in MC3T3-E1 cells. Further experiments revealed that LIPUS stimulated osteogenic differentiation in the presence of smoothened agonist (SAG) treatment. These results indicate that LIPUS stimulates osteogenic differentiation and the maturation of osteoblasts by a primary cilium-mediated activation of hedgehog signaling.
巻・号 119(6)
ページ 4352-4360
公開日 2018-6-1
DOI 10.1002/jcb.26418
PMID 28981158
MeSH Animals Cell Differentiation* Hedgehog Proteins / metabolism* Male Mice Osteoblasts / cytology Osteoblasts / metabolism* Signal Transduction* Ultrasonic Waves* Zinc Finger Protein GLI1 / metabolism Zinc Finger Protein Gli2 / metabolism
IF 3.448
引用数 8
リソース情報
ヒト・動物細胞 MC3T3-E1(RCB1126)