RRC ID |
49319
|
著者 |
Resmini G, Rizzo S, Franchin C, Zanin R, Penzo C, Pegoraro S, Ciani Y, Piazza S, Arrigoni G, Sgarra R, Manfioletti G.
|
タイトル |
HMGA1 regulates the Plasminogen activation system in the secretome of breast cancer cells.
|
ジャーナル |
Sci Rep
|
Abstract |
Cancer cells secrete proteins that modify the extracellular environment acting as autocrine and paracrine stimulatory factors and have a relevant role in cancer progression. The HMGA1 oncofetal protein has a prominent role in controlling the expression of an articulated set of genes involved in various aspect of cancer cell transformation. However, little is known about its role in influencing the secretome of cancer cells. Performing an iTRAQ LC-MS/MS screening for the identification of secreted proteins, in an inducible model of HMGA1 silencing in breast cancer cells, we found that HMGA1 has a profound impact on cancer cell secretome. We demonstrated that the pool of HMGA1-linked secreted proteins has pro-migratory and pro-invasive stimulatory roles. From an inspection of the HMGA1-dependent secreted factors it turned out that HMGA1 influences the presence in the extra cellular milieu of key components of the Plasminogen activation system (PLAU, SERPINE1, and PLAUR) that has a prominent role in promoting metastasis, and that HMGA1 has a direct role in regulating the transcription of two of them, i.e. PLAU and SERPINE1. The ability of HMGA1 to regulate the plasminogen activator system may constitute an important mechanism by which HMGA1 promotes cancer progression.
|
巻・号 |
7(1)
|
ページ |
11768
|
公開日 |
2017-9-18
|
DOI |
10.1038/s41598-017-11409-4
|
PII |
10.1038/s41598-017-11409-4
|
PMID |
28924209
|
PMC |
PMC5603555
|
MeSH |
Cell Line, Tumor
Cell Transformation, Neoplastic*
Female
Gene Expression Regulation, Neoplastic*
HEK293 Cells
HMGA Proteins / metabolism*
Humans
Neoplasm Proteins / genetics
Neoplasm Proteins / metabolism*
Plasminogen / genetics
Plasminogen / metabolism*
Triple Negative Breast Neoplasms / genetics
Triple Negative Breast Neoplasms / metabolism*
Triple Negative Breast Neoplasms / pathology
|
IF |
3.998
|
引用数 |
15
|
リソース情報 |
遺伝子材料 |
pGL4-phPLAU (RDB07487)
pGL4-phSERPINE1(PAI-1) (RDB07461). |