RRC ID 47277
著者 Wang M, Chen PY, Wang CH, Lai TT, Tsai PI, Cheng YJ, Kao HH, Chien CT.
タイトル Dbo/Henji Modulates Synaptic dPAK to Gate Glutamate Receptor Abundance and Postsynaptic Response.
ジャーナル PLoS Genet
Abstract In response to environmental and physiological changes, the synapse manifests plasticity while simultaneously maintains homeostasis. Here, we analyzed mutant synapses of henji, also known as dbo, at the Drosophila neuromuscular junction (NMJ). In henji mutants, NMJ growth is defective with appearance of satellite boutons. Transmission electron microscopy analysis indicates that the synaptic membrane region is expanded. The postsynaptic density (PSD) houses glutamate receptors GluRIIA and GluRIIB, which have distinct transmission properties. In henji mutants, GluRIIA abundance is upregulated but that of GluRIIB is not. Electrophysiological results also support a GluR compositional shift towards a higher IIA/IIB ratio at henji NMJs. Strikingly, dPAK, a positive regulator for GluRIIA synaptic localization, accumulates at the henji PSD. Reducing the dpak gene dosage suppresses satellite boutons and GluRIIA accumulation at henji NMJs. In addition, dPAK associated with Henji through the Kelch repeats which is the domain essential for Henji localization and function at postsynapses. We propose that Henji acts at postsynapses to restrict both presynaptic bouton growth and postsynaptic GluRIIA abundance by modulating dPAK.
巻・号 12(10)
ページ e1006362
公開日 2016-10-1
DOI 10.1371/journal.pgen.1006362
PII PGENETICS-D-16-00901
PMID 27736876
PMC PMC5065118
MeSH Animals Cell Adhesion Molecules, Neuronal / genetics Drosophila Proteins / genetics* Drosophila melanogaster / genetics Drosophila melanogaster / ultrastructure Kelch Repeat / genetics Microscopy, Electron, Transmission Neuromuscular Junction / genetics* Neuromuscular Junction / ultrastructure Presynaptic Terminals / metabolism Receptors, Glutamate / genetics* Receptors, Ionotropic Glutamate / genetics* Synapses / genetics* Synapses / ultrastructure Synaptic Transmission / genetics p21-Activated Kinases / genetics*
IF 5.175
引用数 3
WOS 分野 GENETICS & HEREDITY
リソース情報
ショウジョウバエ 5562R-1 5562R-3