RRC ID 49293
著者 Vaccaro A, Issa AR, Seugnet L, Birman S, Klarsfeld A.
タイトル Drosophila Clock Is Required in Brain Pacemaker Neurons to Prevent Premature Locomotor Aging Independently of Its Circadian Function.
ジャーナル PLoS Genet
Abstract Circadian clocks control many self-sustained rhythms in physiology and behavior with approximately 24-hour periodicity. In many organisms, oxidative stress and aging negatively impact the circadian system and sleep. Conversely, loss of the clock decreases resistance to oxidative stress, and may reduce lifespan and speed up brain aging and neurodegeneration. Here we examined the effects of clock disruptions on locomotor aging and longevity in Drosophila. We found that lifespan was similarly reduced in three arrhythmic mutants (ClkAR, cyc0 and tim0) and in wild-type flies under constant light, which stops the clock. In contrast, ClkAR mutants showed significantly faster age-related locomotor deficits (as monitored by startle-induced climbing) than cyc0 and tim0, or than control flies under constant light. Reactive oxygen species accumulated more with age in ClkAR mutant brains, but this did not appear to contribute to the accelerated locomotor decline of the mutant. Clk, but not Cyc, inactivation by RNA interference in the pigment-dispersing factor (PDF)-expressing central pacemaker neurons led to similar loss of climbing performance as ClkAR. Conversely, restoring Clk function in these cells was sufficient to rescue the ClkAR locomotor phenotype, independently of behavioral rhythmicity. Accelerated locomotor decline of the ClkAR mutant required expression of the PDF receptor and correlated to an apparent loss of dopaminergic neurons in the posterior protocerebral lateral 1 (PPL1) clusters. This neuronal loss was rescued when the ClkAR mutation was placed in an apoptosis-deficient background. Impairing dopamine synthesis in a single pair of PPL1 neurons that innervate the mushroom bodies accelerated locomotor decline in otherwise wild-type flies. Our results therefore reveal a novel circadian-independent requirement for Clk in brain circadian neurons to maintain a subset of dopaminergic cells and avoid premature locomotor aging in Drosophila.
巻・号 13(1)
ページ e1006507
公開日 2017-1-1
DOI 10.1371/journal.pgen.1006507
PII PGENETICS-D-16-01262
PMID 28072817
PMC PMC5224980
MeSH ARNTL Transcription Factors / genetics ARNTL Transcription Factors / metabolism Aging / genetics* Animals Brain / cytology Brain / growth & development Brain / metabolism CLOCK Proteins / genetics* CLOCK Proteins / metabolism Circadian Rhythm / genetics* Dopaminergic Neurons / metabolism* Drosophila / genetics* Drosophila / growth & development Drosophila Proteins / genetics* Drosophila Proteins / metabolism Locomotion / genetics* Receptors, G-Protein-Coupled / genetics Receptors, G-Protein-Coupled / metabolism
IF 5.175
引用数 24
WOS 分野 GENETICS & HEREDITY
リソース情報
ショウジョウバエ 7391R-3