RRC ID 47294
著者 Lence T, Akhtar J, Bayer M, Schmid K, Spindler L, Ho CH, Kreim N, Andrade-Navarro MA, Poeck B, Helm M, Roignant JY.
タイトル m6A modulates neuronal functions and sex determination in Drosophila.
ジャーナル Nature
Abstract N6-methyladenosine RNA (m6A) is a prevalent messenger RNA modification in vertebrates. Although its functions in the regulation of post-transcriptional gene expression are beginning to be unveiled, the precise roles of m6A during development of complex organisms remain unclear. Here we carry out a comprehensive molecular and physiological characterization of the individual components of the methyltransferase complex, as well as of the YTH domain-containing nuclear reader protein in Drosophila melanogaster. We identify the member of the split ends protein family, Spenito, as a novel bona fide subunit of the methyltransferase complex. We further demonstrate important roles of this complex in neuronal functions and sex determination, and implicate the nuclear YT521-B protein as a main m6A effector in these processes. Altogether, our work substantially extends our knowledge of m6A biology, demonstrating the crucial functions of this modification in fundamental processes within the context of the whole animal.
巻・号 540(7632)
ページ 242-247
公開日 2016-12-8
DOI 10.1038/nature20568
PII nature20568
PMID 27919077
MeSH Adenosine / analogs & derivatives* Adenosine / metabolism Alternative Splicing Animals Behavior, Animal / physiology Drosophila Proteins / chemistry Drosophila Proteins / deficiency Drosophila Proteins / genetics Drosophila Proteins / metabolism Drosophila melanogaster / cytology Drosophila melanogaster / enzymology Drosophila melanogaster / physiology* Female Male Methyltransferases / chemistry Methyltransferases / metabolism Nervous System / metabolism Neurons / physiology* Nuclear Proteins / chemistry Nuclear Proteins / deficiency Nuclear Proteins / genetics Nuclear Proteins / metabolism Phenotype Protein Subunits / chemistry Protein Subunits / metabolism RNA-Binding Proteins / genetics Sex Determination Processes / genetics Sex Determination Processes / physiology*
IF 42.779
引用数 172
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ショウジョウバエ pBFv-U6.2