RRC ID 38370
著者 Inobe T, Nozaki M, Nukina N.
タイトル Artificial regulation of p53 function by modulating its assembly.
ジャーナル Biochem Biophys Res Commun
Abstract The tumor suppressor p53, a 393-amino acid transcription factor with four domains, induces cell cycle arrest, senescence, and apoptosis in response to diverse stress. Tetramer formation is critical for the function of p53. The tetramerization domain permits the tetramerization of p53, where bundled four DNA-binding domains recognize the specific target DNA sequences and activate hundreds of genes, which lead to the various cell fates. Here we show that tumor suppressive functions of p53 can be regulated by manipulating tetramer formation of an engineered p53, in which tetramerization domain of p53 is replaced with an inducible tetramer forming protein. This result suggests that artificial regulation of p53 activity by the engineered p53 is a useful tool to investigate the tumor suppression mechanism of p53 and to combat cancer.
巻・号 467(2)
ページ 322-7
公開日 2015-11-13
DOI 10.1016/j.bbrc.2015.09.162
PII S0006-291X(15)30694-X
PMID 26454170
MeSH Escherichia coli / genetics Escherichia coli / metabolism Gene Expression Humans Plasmids / chemistry Plasmids / metabolism Protein Engineering Protein Interaction Domains and Motifs Protein Multimerization Recombinant Fusion Proteins / chemistry* Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism TOR Serine-Threonine Kinases / chemistry* TOR Serine-Threonine Kinases / genetics TOR Serine-Threonine Kinases / metabolism Tacrolimus Binding Proteins / chemistry* Tacrolimus Binding Proteins / genetics Tacrolimus Binding Proteins / metabolism Tumor Suppressor Protein p53 / chemistry* Tumor Suppressor Protein p53 / genetics Tumor Suppressor Protein p53 / metabolism
IF 2.985
引用数 5
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 Saos-2(RCB3688)