RRC ID 48691
著者 Hirashima T, Toyoshima M, Moriyama T, Nakamura Y, Sato N.
タイトル Characterization of phosphoethanolamine-N-methyltransferases in green algae.
ジャーナル Biochem Biophys Res Commun
Abstract Phosphatidylcholine (PtdCho) is a common and abundant phospholipid in most eukaryotic organisms. Although it has been known that the model green alga Chlamydomonas reinhardtii lacks PtdCho, we recently detected PtdCho in four Chlamydomonas species. Homology search of draft genomic sequences of the four PtdCho-containing algae suggested existence of phosphoethanolamine-N-methyltransferase (PEAMT) in C. applanata and C. asymmetrica, which is the key enzyme in PtdCho biosynthesis in land plants. Here we analyzed the putative genes encoding PEAMT in C. applanata and C. asymmetrica, named CapPEAMT and CasPEAMT, respectively. In vitro assays with recombinant CapPEAMT and CasPEAMT indicated that they have the methylation activity for phosphoethanolamine, but not the methylation activity for phosphomonomethylethanolamine, in contrast with land plant PEAMTs, that possess the three successive methylation activities.
巻・号 488(1)
ページ 141-146
公開日 2017-6-17
DOI 10.1016/j.bbrc.2017.05.026
PII S0006-291X(17)30872-0
PMID 28483522
MeSH Amino Acid Sequence Chlorophyta / cytology Chlorophyta / enzymology* Chlorophyta / metabolism Phosphatidylethanolamine N-Methyltransferase / chemistry* Phosphatidylethanolamine N-Methyltransferase / genetics Phosphatidylethanolamine N-Methyltransferase / metabolism* Sequence Alignment
IF 2.985
引用数 4
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY BIOPHYSICS
リソース情報
藻類 NIES-2202 NIES-2207 NIES-2212 NIES-2235 NIES-2242