RRC ID 36830
著者 González-Barrios M, Fierro-González JC, Krpelanova E, Mora-Lorca JA, Pedrajas JR, Peñate X, Chavez S, Swoboda P, Jansen G, Miranda-Vizuete A.
タイトル Cis- and trans-regulatory mechanisms of gene expression in the ASJ sensory neuron of Caenorhabditis elegans.
ジャーナル Genetics
Abstract The identity of a given cell type is determined by the expression of a set of genes sharing common cis-regulatory motifs and being regulated by shared transcription factors. Here, we identify cis and trans regulatory elements that drive gene expression in the bilateral sensory neuron ASJ, located in the head of the nematode Caenorhabditis elegans. For this purpose, we have dissected the promoters of the only two genes so far reported to be exclusively expressed in ASJ, trx-1 and ssu-1. We hereby identify the ASJ motif, a functional cis-regulatory bipartite promoter region composed of two individual 6 bp elements separated by a 3 bp linker. The first element is a 6 bp CG-rich sequence that presumably binds the Sp family member zinc-finger transcription factor SPTF-1. Interestingly, within the C. elegans nervous system SPTF-1 is also found to be expressed only in ASJ neurons where it regulates expression of other genes in these neurons and ASJ cell fate. The second element of the bipartite motif is a 6 bp AT-rich sequence that is predicted to potentially bind a transcription factor of the homeobox family. Together, our findings identify a specific promoter signature and SPTF-1 as a transcription factor that functions as a terminal selector gene to regulate gene expression in C. elegans ASJ sensory neurons.
巻・号 200(1)
ページ 123-34
公開日 2015-5-1
DOI 10.1534/genetics.115.176172
PII genetics.115.176172
PMID 25769980
PMC PMC4423358
MeSH Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism GC Rich Sequence Promoter Regions, Genetic* Sensory Receptor Cells / metabolism* Sulfotransferases / genetics Sulfotransferases / metabolism Thioredoxins / genetics Thioredoxins / metabolism Transcription Factors / genetics Transcription Factors / metabolism Transcriptional Activation*
IF 4.015
引用数 7
WOS 分野 GENETICS & HEREDITY
リソース情報
線虫 tm1130 tm784