RRC ID 35614
著者 Reyes LH, Abdelaal AS, Kao KC.
タイトル Genetic determinants for n-butanol tolerance in evolved Escherichia coli mutants: cross adaptation and antagonistic pleiotropy between n-butanol and other stressors.
ジャーナル Appl Environ Microbiol
Abstract Cross-tolerance and antagonistic pleiotropy have been observed between different complex phenotypes in microbial systems. These relationships between adaptive landscapes are important for the design of industrially relevant strains, which are generally subjected to multiple stressors. In our previous work, we evolved Escherichia coli for enhanced tolerance to the biofuel n-butanol and discovered a molecular mechanism of n-butanol tolerance that also conferred tolerance to the cationic antimicrobial peptide polymyxin B in one specific lineage (green fluorescent protein [GFP] labeled) in the evolved population. In this work, we aim to identify additional mechanisms of n-butanol tolerance in an independent lineage (yellow fluorescent protein [YFP] labeled) from the same evolved population and to further explore potential cross-tolerance and antagonistic pleiotropy between n-butanol tolerance and other industrially relevant stressors. Analysis of the transcriptome data of the YFP-labeled mutants allowed us to discover additional membrane-related and osmotic stress-related genes that confer n-butanol tolerance in E. coli. Interestingly, the n-butanol resistance mechanisms conferred by the membrane-related genes appear to be specific to n-butanol and are in many cases antagonistic with isobutanol and ethanol. Furthermore, the YFP-labeled mutants showed cross-tolerance between n-butanol and osmotic stress, while the GFP-labeled mutants showed antagonistic pleiotropy between n-butanol and osmotic stress tolerance.
巻・号 79(17)
ページ 5313-20
公開日 2013-9-1
DOI 10.1128/AEM.01703-13
PII AEM.01703-13
PMID 23811509
PMC PMC3753956
MeSH 1-Butanol / toxicity* Bacterial Proteins / genetics Butanols / toxicity Cell Membrane / drug effects* Cell Membrane / genetics* Drug Tolerance* Escherichia coli / drug effects* Escherichia coli / genetics* Ethanol / toxicity Gene Expression Profiling Luminescent Proteins / genetics Osmotic Pressure* Staining and Labeling Stress, Physiological
IF 4.016
引用数 29
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
リソース情報
原核生物(大腸菌) ASKA collection