RRC ID 25693
著者 Mizuno N, Nitta M, Sato K, Nasuda S.
タイトル A wheat homologue of PHYTOCLOCK 1 is a candidate gene conferring the early heading phenotype to einkorn wheat.
ジャーナル Genes Genet Syst
Abstract An X-ray mutant showing an early flowering phenotype has been identified in einkorn wheat (Triticum monococcum L.), for which a major QTL for heading time was previously mapped in the telomeric region on the long arm of chromosome 3A. Recent advances in Triticeae genomics revealed that the gene order in this region is highly conserved between wheat and barley. Thus, we adopted a hypothetical gene order in barley, the so-called GenomeZipper, to develop DNA markers for fine mapping the target gene in wheat. We identified three genes tightly linked to the early heading phenotype. PCR analysis revealed that early-flowering is associated with the deletion of two genes in the mutant. Of the two deleted genes, one is an ortholog of the LUX ARRHYTHMO (LUX)/PHYTOCLOCK 1 (PCL1) gene found in Arabidopsis, which regulates the circadian clock and flowering time. We found distorted expression patterns of two clock genes (TOC1 and LHY) in the einkorn pcl1 deletion mutant as was reported for the Arabidopsis lux mutant. Transcript accumulation levels of photoperiod-response related genes, a photoperiod sensitivity gene (Ppd-1) and two wheat CONSTANS-like genes (WCO1 and TaHd1), were significantly higher in the einkorn wheat mutant. In addition, transcripts of the wheat florigen gene (WFT) accumulated temporally under short-day conditions in the einkorn wheat mutant. These results suggest that deletion of WPCL1 leads to abnormally higher expression of Ppd-1, resulting in the accumulation of WFT transcripts that triggers flowering even under short-day conditions. Our observations from gene mapping, gene deletions, and expression levels of flowering related genes strongly suggest that WPCL1 is the most likely candidate gene for controlling the early flowering phenotype in the einkorn wheat mutant.
巻・号 87(6)
ページ 357-67
公開日 2012-1-1
DOI 10.1266/ggs.87.357
PII DN/JST.JSTAGE/ggs/87.357
PMID 23558642
MeSH Arabidopsis / genetics Arabidopsis Proteins / genetics* Chromosome Mapping Circadian Rhythm / genetics Circadian Rhythm / radiation effects Circadian Rhythm Signaling Peptides and Proteins / genetics* DNA-Binding Proteins / genetics Flowers / genetics* Gene Expression Regulation, Plant / radiation effects* Hordeum / genetics Mutation / radiation effects* RNA, Messenger / genetics* Sequence Homology, Amino Acid Transcription Factors / genetics* Triticum / genetics* Triticum / radiation effects X-Rays
IF 0.917
引用数 22
WOS 分野 GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
オオムギ オオムギEST
コムギ KT003-005 KT003-001 RILWA1