RRC ID 48133
著者 Xu WM, Xu JQ, Zhou Y, Li Y, Lü ZZ, Hou J, Zhu L, Cui HL.
タイトル Halomarina salina sp. nov., isolated from a marine solar saltern.
ジャーナル Antonie Van Leeuwenhoek
Abstract A halophilic archaeal strain, designated ZS-57-S(T), was isolated from Zhoushan marine solar saltern, China. Cells were observed to be pleomorphic, stained Gram-negative and formed red pigmented colonies on agar plates. Optimal growth was obtained at 3.9 M NaCl (range 1.4-4.8 M), 0.3 M MgCl2 (range 0-1.0 M), 30 °C (range 20-55 °C) and pH 6.5-7.5 (range 5.5-9.0). The cells were found to lyse in distilled water and the minimal NaCl concentration to prevent cell lysis was determined to be 5 % (w/v). The major polar lipids were identified as C20C20 and C20C25 diether derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate, glucosyl mannosyl glucosyl diether and two unidentified glycolipids. The 16S rRNA gene and rpoB' gene of strain ZS-57-S(T) were phylogenetically related to the corresponding genes of Halomarina oriensis JCM 16495(T) (98.2 and 93.7 % similarities, respectively). The DNA G+C content of strain ZS-57-S(T) was determined to be 67.1 mol% (T m). The phenotypic, chemotaxonomic and phylogenetic properties suggested that strain ZS-57-S(T) (=CGMCC 1.12543(T) = JCM 30039(T)) represents a new species of the genus Halomarina, for which the name Halomarina salina sp. nov. is proposed.
巻・号 109(8)
ページ 1121-6
公開日 2016-8-1
DOI 10.1007/s10482-016-0714-5
PII 10.1007/s10482-016-0714-5
PMID 27198657
MeSH China Genome, Archaeal Halobacteriaceae / classification* Halobacteriaceae / genetics Halobacteriaceae / isolation & purification* Halobacteriaceae / metabolism Phospholipids / metabolism Phylogeny Pigments, Biological / metabolism Salinity Seawater / microbiology* Sequence Analysis, DNA
IF 2.674
引用数 4
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 16495 JCM 30039