RRC ID 48146
著者 Liu Y, Zhao R, Wang R, Yao S, Zhai L, Zhang X, Chen C, Cao Y, Xu T, Ge Y, Zhao J, Cheng C.
タイトル Paenibacillus chinensis sp. nov., isolated from maize (Zea mays L.) seeds.
ジャーナル Antonie Van Leeuwenhoek
Abstract Four Gram-stain positive bacterial strains, designated as 4R1(T), 4R9, 4L13 and 4L18, isolated from seeds of hybrid maize (Zea mays L., Jingke 968), were investigated using a polyphasic taxonomic approach. The cells were found to be facultatively aerobic, motile, spore-forming and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolates should be recognised as a species of the genus Paenibacillus, with two close neighbours being Paenibacillus nicotianae YIM h-19(T) (98.41 % similarity) and Paenibacillus hordei RH-N24(T) (98.37 %). The DNA G+C content of strain 4R1(T) was determined to be 51.6 mol %. Its polar lipid profile was found to consist of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified lipid. The predominant respiratory quinone was identified as MK-7 and the major fatty acids were found to be anteiso-C15:0, anteiso-C12:0, anteiso-C13:0 and anteiso-C11:0. Strains 4R1(T), 4R9, 4L13 and 4L18 were clearly distinguished from the reference type strains using phylogenetic analysis, DNA-DNA hybridization and a range of physiological and biochemical characteristics. It is evident from the genotypic and phenotypic data that strains 4R1(T), 4R9, 4L13 and 4L18 represent a novel species of the genus Paenibacillus, for which the name Paenibacillus chinensis sp. nov. is proposed. The type strain is 4R1(T) (=KCTC 33672(T) = CICC 23864(T)).
巻・号 109(2)
ページ 207-13
公開日 2016-2-1
DOI 10.1007/s10482-015-0622-0
PII 10.1007/s10482-015-0622-0
PMID 26589682
MeSH Base Composition DNA, Bacterial / genetics Fatty Acids / chemistry Fatty Acids / metabolism Molecular Sequence Data Paenibacillus / classification Paenibacillus / genetics Paenibacillus / isolation & purification* Paenibacillus / metabolism Phylogeny RNA, Ribosomal, 16S / genetics Seeds / microbiology* Zea mays / microbiology*
IF 2.674
引用数 5
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 17570