RRC ID 40911
著者 Inoue J, Oshima K, Suda W, Sakamoto M, Iino T, Noda S, Hongoh Y, Hattori M, Ohkuma M.
タイトル Distribution and evolution of nitrogen fixation genes in the phylum Bacteroidetes.
ジャーナル Microbes Environ
Abstract Diazotrophs had not previously been identified among bacterial species in the phylum Bacteroidetes until the rapid expansion of bacterial genome sequences, which revealed the presence of nitrogen fixation (nif) genes in this phylum. We herein determined the draft genome sequences of Bacteroides graminisolvens JCM 15093(T) and Geofilum rubicundum JCM 15548(T). In addition to these and previously reported 'Candidatus Azobacteroides pseudotrichonymphae' and Paludibacter propionicigenes, an extensive survey of the genome sequences of diverse Bacteroidetes members revealed the presence of a set of nif genes (nifHDKENB) in strains of Dysgonomonas gadei, Dysgonomonas capnocytophagoides, Saccharicrinis fermentans, and Alkaliflexus imshenetskii. These eight species belonged to and were distributed sporadically within the order Bacteroidales. Acetylene reduction activity was detected in the five species examined, strongly suggesting their diazotrophic nature. Phylogenetic analyses showed monophyletic clustering of the six Nif protein sequences in the eight Bacteroidales species, implying that nitrogen fixation is ancestral to Bacteroidales and has been retained in these species, but lost in many other lineages. The identification of nif genes in Bacteroidales facilitates the prediction of the organismal origins of related sequences directly obtained from various environments.
巻・号 30(1)
ページ 44-50
公開日 2015-1-1
DOI 10.1264/jsme2.ME14142
PMID 25736980
PMC PMC4356463
MeSH Acetylene / metabolism Bacteroidetes / classification Bacteroidetes / genetics* Bacteroidetes / metabolism* Cluster Analysis DNA, Bacterial / chemistry DNA, Bacterial / genetics Evolution, Molecular* Genome, Bacterial Metabolic Networks and Pathways / genetics* Molecular Sequence Data Nitrogen Fixation* Oxidation-Reduction Phylogeny Sequence Analysis, DNA Sequence Homology
IF 2.575
引用数 20
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
リソース情報
一般微生物 JCM 15093   JCM 15548 JCM 21142 JCM 1408 JCM 16698 JCM 13257 JCM 17038 JCM 13650