RRC ID |
30789
|
著者 |
Jarsch IK, Konrad SS, Stratil TF, Urbanus SL, Szymanski W, Braun P, Braun KH, Ott T.
|
タイトル |
Plasma Membranes Are Subcompartmentalized into a Plethora of Coexisting and Diverse Microdomains in Arabidopsis and Nicotiana benthamiana.
|
ジャーナル |
Plant Cell
|
Abstract |
Eukaryotic plasma membranes are highly compartmentalized structures. So far, only a few individual proteins that function in a wide range of cellular processes have been shown to segregate into microdomains. However, the biological roles of most microdomain-associated proteins are unknown. Here, we investigated the heterogeneity of distinct microdomains and the complexity of their coexistence. This diversity was determined in living cells of intact multicellular tissues using 20 different marker proteins from Arabidopsis thaliana, mostly belonging to the Remorin protein family. These proteins associate with microdomains at the cytosolic leaflet of the plasma membrane. We characterized these membrane domains and determined their lateral dynamics by extensive quantitative image analysis. Systematic colocalization experiments with an extended subset of marker proteins tested in 45 different combinations revealed the coexistence of highly distinct membrane domains on individual cell surfaces. These data provide valuable tools to study the lateral segregation of membrane proteins and their biological functions in living plant cells. They also demonstrate that widely used biochemical approaches such as detergent-resistant membranes cannot resolve this biological complexity of membrane compartmentalization in vivo.
|
巻・号 |
26(4)
|
ページ |
1698-1711
|
公開日 |
2014-4-1
|
DOI |
10.1105/tpc.114.124446
|
PII |
tpc.114.124446
|
PMID |
24714763
|
PMC |
PMC4036580
|
IF |
9.618
|
引用数 |
81
|
WOS 分野
|
PLANT SCIENCES
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
リソース情報 |
シロイヌナズナ / 植物培養細胞・遺伝子 |
pda05122
pda13433
pda00891
pda11840
pda01425
pda12816
pdx96365 |