RRC ID 6169
著者 Frei dit Frey N, Muller P, Jammes F, Kizis D, Leung J, Perrot-Rechenmann C, Bianchi MW.
タイトル The RNA binding protein Tudor-SN is essential for stress tolerance and stabilizes levels of stress-responsive mRNAs encoding secreted proteins in Arabidopsis.
ジャーナル Plant Cell
Abstract Tudor-SN (TSN) copurifies with the RNA-induced silencing complex in animal cells where, among other functions, it is thought to act on mRNA stability via the degradation of specific dsRNA templates. In plants, TSN has been identified biochemically as a cytoskeleton-associated RNA binding activity. In eukaryotes, it has recently been identified as a conserved primary target of programmed cell death-associated proteolysis. We have investigated the physiological role of TSN by isolating null mutations for two homologous genes in Arabidopsis thaliana. The double mutant tsn1 tsn2 displays only mild growth phenotypes under nonstress conditions, but germination, growth, and survival are severely affected under high salinity stress. Either TSN1 or TSN2 alone can complement the double mutant, indicating their functional redundancy. TSN accumulates heterogeneously in the cytosol and relocates transiently to a diffuse pattern in response to salt stress. Unexpectedly, stress-regulated mRNAs encoding secreted proteins are significantly enriched among the transcripts that are underrepresented in tsn1 tsn2. Our data also reveal that TSN is important for RNA stability of its targets. These findings show that TSN is essential for stress tolerance in plants and implicate TSN in new, potentially conserved mechanisms acting on mRNAs entering the secretory pathway.
巻・号 22(5)
ページ 1575-91
公開日 2010-5-1
DOI 10.1105/tpc.109.070680
PII tpc.109.070680
PMID 20484005
PMC PMC2899877
MeSH Adaptation, Physiological / drug effects Adaptation, Physiological / genetics* Arabidopsis / cytology Arabidopsis / drug effects Arabidopsis / genetics* Arabidopsis / growth & development Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Cytoplasm / drug effects Cytoplasm / metabolism Gene Expression Profiling Gene Expression Regulation, Plant* / drug effects Genes, Plant / genetics Genetic Complementation Test Molecular Sequence Data Mutation / genetics Plant Roots / cytology Plant Roots / drug effects Plant Roots / genetics Plant Roots / growth & development Protein Transport / drug effects RNA Stability / drug effects RNA Stability / genetics* RNA, Messenger / genetics RNA, Messenger / metabolism RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism* RNA-Induced Silencing Complex / metabolism Seeds / drug effects Seeds / genetics Sodium Chloride / pharmacology Soil Stress, Physiological / drug effects Stress, Physiological / genetics*
IF 9.618
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda06063 pda12297