RRC ID 45628
著者 Prevo B, Mangeol P, Oswald F, Scholey JM, Peterman EJ.
タイトル Functional differentiation of cooperating kinesin-2 motors orchestrates cargo import and transport in C. elegans cilia.
ジャーナル Nat Cell Biol
Abstract Intracellular transport depends on cooperation between distinct motor proteins. Two anterograde intraflagellar transport (IFT) motors, heterotrimeric kinesin-II and homodimeric OSM-3, cooperate to move cargo along Caenorhabditis elegans cilia. Here, using quantitative fluorescence microscopy, with single-molecule sensitivity, of IFT in living strains containing single-copy transgenes encoding fluorescent IFT proteins, we show that kinesin-II transports IFT trains through the ciliary base and transition zone to a 'handover zone' on the proximal axoneme. There, OSM-3 gradually replaces kinesin-II, yielding velocity profiles inconsistent with in vitro motility assays, and then drives transport to the ciliary tip. Dissociated kinesin-II motors undergo rapid turnaround and recycling to the ciliary base, whereas OSM-3 is recycled mainly to the handover zone. This reveals a functional differentiation in which the slower, less processive kinesin-II imports IFT trains into the cilium and OSM-3 drives their long-range transport, thereby optimizing cargo delivery.
巻・号 17(12)
ページ 1536-45
公開日 2015-12-1
DOI 10.1038/ncb3263
PII ncb3263
PMID 26523365
MeSH A Kinase Anchor Proteins / genetics A Kinase Anchor Proteins / metabolism Algorithms Animals Biological Transport / genetics Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cilia / metabolism* Computer Simulation Kinesins / chemistry Kinesins / genetics Kinesins / metabolism* Kinetics Luminescent Proteins / genetics Luminescent Proteins / metabolism Microscopy, Fluorescence Models, Biological Mutation Protein Multimerization Video Recording
IF 20.042
引用数 70
WOS 分野 CELL BIOLOGY
リソース情報
線虫 tm3433