RRC ID |
2515
|
著者 |
Saraike T, Shitsukawa N, Yamamoto Y, Hagita H, Iwasaki Y, Takumi S, Murai K.
|
タイトル |
Identification of a protein kinase gene associated with pistillody, homeotic transformation of stamens into pistil-like structures, in alloplasmic wheat.
|
ジャーナル |
Planta
|
Abstract |
Homeotic transformation of stamens into pistil-like structures (called pistillody) has been reported in cytoplasmic substitution (alloplasmic) lines of bread wheat (Triticum aestivum) having the cytoplasm of a wild relative species, Aegilops crassa. Our previous studies indicated that pistillody is caused by alterations of the class B MADS-box gene expression pattern associated with mitochondrial gene(s) in the Ae. crassa cytoplasm. To elucidate the nuclear gene involved in the cross-talk between pistillody-related mitochondrial gene(s) and nuclear homeotic genes, we performed cDNA subtraction analysis using cDNAs derived from young spikes of a pistillody line and a normal line. As a result, we identified a protein kinase gene, WPPK1 (wheat pistillody-related protein kinase 1), which is upregulated in the young spikes of the pistillody line. RT-PCR analysis indicated that WPPK1 is strongly expressed in pistils and pistil-like stamens in the pistillody line, suggesting that it is involved in the formation of pistil-like stamens as well as pistils. The full-length cDNA sequence for WPPK1 showed high similarity with a flowering plant PVPK-1 protein kinase, and phylogenetic analysis indicated that it is a member of AGC group protein kinases. Furthermore, a phosphorylation assay indicated that it has protein kinase activity. In situ hybridization analysis revealed that WPPK1 is expressed in developing pistils and pistil-like stamens as well as in their primordia. These indicate that in the alloplasmic line, WPPK1 plays a role in formation and development of pistil-like stamens.
|
巻・号 |
227(1)
|
ページ |
211-21
|
公開日 |
2007-12-1
|
DOI |
10.1007/s00425-007-0608-x
|
PMID |
17704940
|
MeSH |
Amino Acid Sequence
Blotting, Western
Flowers / enzymology
Flowers / genetics*
Flowers / growth & development
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
In Situ Hybridization
Molecular Sequence Data
Phylogeny
Plant Proteins / genetics*
Plant Proteins / metabolism
Protein Kinases / genetics*
Protein Kinases / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Sequence Homology, Amino Acid
Triticum / enzymology
Triticum / genetics*
Triticum / growth & development
|
IF |
3.39
|
引用数 |
15
|
WOS 分野
|
PLANT SCIENCES
|
リソース情報 |
コムギ |
CS
7BS [(cr)-CSdt7BS]? |