RRC ID |
29117
|
著者 |
Morita Y, Takagi K, Fukuchi-Mizutani M, Ishiguro K, Tanaka Y, Nitasaka E, Nakayama M, Saito N, Kagami T, Hoshino A, Iida S.
|
タイトル |
A chalcone isomerase-like protein enhances flavonoid production and flower pigmentation.
|
ジャーナル |
Plant J
|
Abstract |
Flavonoids are major pigments in plants, and their biosynthetic pathway is one of the best-studied metabolic pathways. Here we have identified three mutations within a gene that result in pale-colored flowers in the Japanese morning glory (Ipomoea nil). As the mutations lead to a reduction of the colorless flavonoid compound flavonol as well as of anthocyanins in the flower petal, the identified gene was designated enhancer of flavonoid production (EFP). EFP encodes a chalcone isomerase (CHI)-related protein classified as a type IV CHI protein. CHI is the second committed enzyme of the flavonoid biosynthetic pathway, but type IV CHI proteins are thought to lack CHI enzymatic activity, and their functions remain unknown. The spatio-temporal expression of EFP and structural genes encoding enzymes that produce flavonoids is very similar. Expression of both EFP and the structural genes is coordinately promoted by genes encoding R2R3-MYB and WD40 family proteins. The EFP gene is widely distributed in land plants, and RNAi knockdown mutants of the EFP homologs in petunia (Petunia hybrida) and torenia (Torenia hybrida) had pale-colored flowers and low amounts of anthocyanins. The flavonol and flavone contents in the knockdown petunia and torenia flowers, respectively, were also significantly decreased, suggesting that the EFP protein contributes in early step(s) of the flavonoid biosynthetic pathway to ensure production of flavonoid compounds. From these results, we conclude that EFP is an enhancer of flavonoid production and flower pigmentation, and its function is conserved among diverse land plant species.
|
巻・号 |
78(2)
|
ページ |
294-304
|
公開日 |
2014-4-1
|
DOI |
10.1111/tpj.12469
|
PMID |
24517863
|
MeSH |
Anthocyanins / chemistry
Anthocyanins / metabolism
Biosynthetic Pathways
Flavonoids / chemistry
Flavonoids / metabolism*
Flowers / anatomy & histology
Flowers / genetics
Flowers / metabolism
Gene Expression Regulation, Plant
Intramolecular Lyases / genetics
Intramolecular Lyases / metabolism
Intramolecular Lyases / physiology*
Ipomoea / anatomy & histology
Ipomoea / genetics
Ipomoea / metabolism
Molecular Sequence Data
Plant Proteins / genetics
Plant Proteins / metabolism
Plant Proteins / physiology*
RNA Interference
|
IF |
6.141
|
引用数 |
47
|
WOS 分野
|
PLANT SCIENCES
|
リソース情報 |
アサガオ |
EST clones
BAC clones
AK1
AK26
AK28
AK31
AK33
AK50
AK60
Q0095
Q0101
Q0153
Q0228
Q0580
Q0584
Q0711
Q0877
Q0912
Q1058
Q1326
Q1327
Q1328
Q1330
Q1332
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Q1334
Q1339
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Q1385
AK112
Q0357
Q0522
Q0589
Q0627
Q0638
Q0778
Q0904
Q0914
Q0096
Q0465
Q0508
Q0543
Q0598
Q0774
Q1035
Q1386 |