RRC ID 15627
著者 Rodrigues DF, Ivanova N, He Z, Huebner M, Zhou J, Tiedje JM.
タイトル Architecture of thermal adaptation in an Exiguobacterium sibiricum strain isolated from 3 million year old permafrost: a genome and transcriptome approach.
ジャーナル BMC Genomics
Abstract BACKGROUND:Many microorganisms have a wide temperature growth range and versatility to tolerate large thermal fluctuations in diverse environments, however not many have been fully explored over their entire growth temperature range through a holistic view of its physiology, genome, and transcriptome. We used Exiguobacterium sibiricum strain 255-15, a psychrotrophic bacterium from 3 million year old Siberian permafrost that grows from -5 degrees C to 39 degrees C to study its thermal adaptation.
RESULTS:The E. sibiricum genome has one chromosome and two small plasmids with a total of 3,015 protein-encoding genes (CDS), and a GC content of 47.7%. The genome and transcriptome analysis along with the organism's known physiology was used to better understand its thermal adaptation. A total of 27%, 3.2%, and 5.2% of E. sibiricum CDS spotted on the DNA microarray detected differentially expressed genes in cells grown at -2.5 degrees C, 10 degrees C, and 39 degrees C, respectively, when compared to cells grown at 28 degrees C. The hypothetical and unknown genes represented 10.6%, 0.89%, and 2.3% of the CDS differentially expressed when grown at -2.5 degrees C, 10 degrees C, and 39 degrees C versus 28 degrees C, respectively.
CONCLUSION:The results show that E. sibiricum is constitutively adapted to cold temperatures stressful to mesophiles since little differential gene expression was observed between 4 degrees C and 28 degrees C, but at the extremities of its Arrhenius growth profile, namely -2.5 degrees C and 39 degrees C, several physiological and metabolic adaptations associated with stress responses were observed.
巻・号 9
ページ 547
公開日 2008-11-18
DOI 10.1186/1471-2164-9-547
PII 1471-2164-9-547
PMID 19019206
PMC PMC2615787
MeSH Acclimatization / genetics* Acclimatization / physiology Amino Acids / metabolism Carbohydrate Metabolism Cold Temperature* DNA Replication Energy Metabolism Gene Expression Profiling Genome, Bacterial / genetics* Gram-Positive Asporogenous Rods / genetics* Gram-Positive Asporogenous Rods / metabolism Gram-Positive Asporogenous Rods / ultrastructure Microscopy, Electron, Transmission Nucleotides / metabolism Siberia Transcription, Genetic
IF 3.594
引用数 75
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY
リソース情報
一般微生物 JCM 13490