RRC ID 48153
著者 Zainal N, Ser HL, Yin WF, Tee KK, Lee LH, Chan KG.
タイトル Streptomyces humi sp. nov., an actinobacterium isolated from soil of a mangrove forest.
ジャーナル Antonie Van Leeuwenhoek
Abstract A novel Streptomyces strain, MUSC 119(T), was isolated from a soil collected from a mangrove forest. Cells of MUSC 119(T) stained Gram-positive and formed light brownish grey aerial mycelium and grayish yellowish brown substrate mycelium on ISP 2 medium. A polyphasic approach was used to determine the taxonomic status of strain MUSC 119(T), which shows a range of phylogenetic and chemotaxonomic properties consistent with those of the genus Streptomyces. The cell wall peptidoglycan consisted of LL-diaminopimelic acid. The predominant menaquinones were identified as MK-9(H8), MK-9(H6) and MK-9(H4). The polar lipid profile consisted of phosphatidylinositol, phosphatidylethanolamine, glycolipids, diphosphatidylglycerol and four phospholipids. The predominant cellular fatty acids were anteiso-C15:0, iso-C16:0, and anteiso-C17:0. The cell wall sugars were glucose, mannose, ribose and rhamnose. The phylogenetic analysis based on 16S rRNA gene sequence similarity showed that strain MUSC119(T) to be closely related to Streptomyces rhizophilus JR-41(T) (99.0 % sequence similarity), S. panaciradicis 1MR-8(T) (98.9 %), S. gramineus JR-43(T) (98.8 %) and S. graminisoli JR-19(T) (98.7 %). These results suggest that MUSC 119(T) should be placed within the genus Streptomyces. DNA-DNA relatedness values between MUSC 119(T) to closely related strains ranged from 14.5 ± 1.3 to 27.5 ± 0.7 %. The G+C content was determined to be 72.6 mol %. The polyphasic study of MUSC 119(T) showed that this strain represents a novel species, for which the name Streptomyces humi sp. nov. is proposed. The type strain of S. humi is MUSC 119(T) (=DSM 42174(T) = MCCC 1K00505(T)).
巻・号 109(3)
ページ 467-74
公開日 2016-3-1
DOI 10.1007/s10482-016-0653-1
PII 10.1007/s10482-016-0653-1
PMID 26786500
MeSH Bacterial Typing Techniques Base Composition Forests* Genome, Bacterial Genomics Phenotype Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Soil Microbiology* Streptomyces / classification* Streptomyces / isolation & purification
IF 2.674
引用数 9
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 4976