RRC ID 33167
著者 Kim W, Kim HD, Jung Y, Kim J, Chung J.
タイトル Drosophila Low Temperature Viability Protein 1 (LTV1) Is Required for Ribosome Biogenesis and Cell Growth Downstream of Drosophila Myc (dMyc).
ジャーナル J Biol Chem
Abstract During animal development, various signaling pathways converge to regulate cell growth. In this study, we identified LTV1 as a novel cell growth regulator in Drosophila. LTV1 mutant larvae exhibited developmental delays and lethality at the second larval stage. Using biochemical studies, we discovered that LTV1 interacted with ribosomal protein S3 and co-purified with free 40S ribosome subunits. We further demonstrated that LTV1 is crucial for ribosome biogenesis through 40S ribosome subunit synthesis and preribosomal RNA processing, suggesting that LTV1 is required for cell growth by regulating protein synthesis. We also demonstrated that Drosophila Myc (dMyc) directly regulates LTV1 transcription and requires LTV1 to stimulate ribosome biogenesis. Importantly, the loss of LTV1 blocked the cell growth and endoreplication induced by dMyc. Combined, these results suggest that LTV1 is a key downstream factor of dMyc-induced cell growth by properly maintaining ribosome biogenesis.
巻・号 290(21)
ページ 13591-604
公開日 2015-5-22
DOI 10.1074/jbc.M114.607036
PII S0021-9258(20)33714-5
PMID 25858587
PMC PMC4505604
MeSH Animals Animals, Genetically Modified / genetics Animals, Genetically Modified / growth & development Animals, Genetically Modified / metabolism* Blotting, Northern Cell Proliferation Cells, Cultured Chromatin Immunoprecipitation Drosophila Proteins / genetics* Drosophila Proteins / metabolism Drosophila melanogaster / genetics Drosophila melanogaster / growth & development Drosophila melanogaster / metabolism* Immunoenzyme Techniques Microscopy, Electron Protein Biosynthesis* Proto-Oncogene Proteins c-myc / genetics Proto-Oncogene Proteins c-myc / metabolism* RNA, Messenger / genetics Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Ribosomes / genetics Ribosomes / metabolism* Temperature
IF 4.238
引用数 5
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ショウジョウバエ CG7686[CB-6202-3] (DGRC#123972)