RRC ID 1723
著者 Saka K, Tadenuma M, Nakade S, Tanaka N, Sugawara H, Nishikawa K, Ichiyoshi N, Kitagawa M, Mori H, Ogasawara N, Nishimura A.
タイトル A complete set of Escherichia coli open reading frames in mobile plasmids facilitating genetic studies.
ジャーナル DNA Res
Abstract To facilitate genetic studies of Escherichia coli, we constructed a complete set of mobile plasmid clones of intact open reading frames (ORFs). Their expression is strictly controlled by Ptac / lacI(q). The plasmids carrying each ORF were introduced into an F+ recA strain and stored in 96-well microtiter plates. In this way, 96 clones can be transferred simultaneously to F- bacteria using the conjugative system. This provides a convenient procedure for systematic identification of ORFs that suppress or complement mutations. We created two types of clone sets: the original set contained individual clones in 45 microtiter plates, and a second set contained pools of 48 clones stored in a single microtiter plate. Using these clone sets, we have identified 403 genes that can correct in trans the temperature-sensitive defect of cell division mutants, which would suggest multiple global regulators for bacterial cell division.
巻・号 12(1)
ページ 63-8
公開日 2005-2-28
DOI 10.1093/dnares/12.1.63
PMID 16106753
MeSH Base Sequence Cell Division / genetics Cloning, Molecular Escherichia coli / genetics* Genes, Bacterial Genetic Complementation Test / methods Models, Genetic Molecular Sequence Data Open Reading Frames / genetics* Plasmids*
IF 4.009
WOS 分野 GENETICS & HEREDITY
リソース情報
情報 E.coli