RRC ID 36030
著者 Hung SS, Wong RC, Sharov AA, Nakatake Y, Yu H, Ko MS.
タイトル Repression of global protein synthesis by Eif1a-like genes that are expressed specifically in the two-cell embryos and the transient Zscan4-positive state of embryonic stem cells.
ジャーナル DNA Res
Abstract Mouse embryonic stem (ES) cells are prototypical stem cells that remain undifferentiated in culture for long periods, yet maintain the ability to differentiate into essentially all cell types. Previously, we have reported that ES cells oscillate between two distinct states, which can be distinguished by the transient expression of Zscan4 genes originally identified for its specific expression in mouse two-cell stage embryos. Here, we report that the nascent protein synthesis is globally repressed in the Zscan4-positive state of ES cells, which is mediated by the transient expression of newly identified eukaryotic translation initiation factor 1A (Eif1a)-like genes. Eif1a-like genes, clustered on Chromosome 12, show the high sequence similarity to the Eifa1 and consist of 10 genes (Eif1al1-Eif1al10) and 9 pseudogenes (Eif1al-ps1-Eif1al-ps9). The analysis of the expressed sequence tag database showed that Eif1a-like genes are expressed mostly in the two-cell stage mouse embryos. Microarray analyses and quantitative real-time polymerase chain reaction analyses show that Eif1a-like genes are expressed specifically in the Zscan4-positive state of ES cells. These results indicate a novel mechanism to repress protein synthesis by Eif1a-like genes and a unique mode of protein synthesis regulation in ES cells, which undergo a transient and reversible repression of global protein synthesis in the Zscan4-positive state.
巻・号 20(4)
ページ 391-402
公開日 2013-8-1
DOI 10.1093/dnares/dst018
PII dst018
PMID 23649898
PMC PMC3738165
MeSH Animals Chromosomes, Mammalian Embryo, Mammalian Embryonic Stem Cells / classification Embryonic Stem Cells / cytology Embryonic Stem Cells / metabolism* Eukaryotic Initiation Factor-1 / classification Eukaryotic Initiation Factor-1 / genetics* Eukaryotic Initiation Factor-1 / metabolism Expressed Sequence Tags Gene Expression Regulation, Developmental* Mice Multigene Family Protein Biosynthesis* Protein Isoforms / classification Protein Isoforms / genetics Protein Isoforms / metabolism Transcription Factors / genetics* Transcription Factors / metabolism
IF 4.009
引用数 17
WOS 分野 GENETICS & HEREDITY
リソース情報
遺伝子材料 pPyCAG-BstXI-IP (RDB07099)