RRC ID 52756
著者 Shah N, Hirakawa H, Kusakabe S, Sandal N, Stougaard J, Schierup MH, Sato S, Andersen SU.
タイトル High-resolution genetic maps of Lotus japonicus and L. burttii based on re-sequencing of recombinant inbred lines.
ジャーナル DNA Res
Abstract Recombinant inbred lines (RILs) derived from bi-parental populations are stable genetic resources, which are widely used for constructing genetic linkage maps. These genetic maps are essential for QTL mapping and can aid contig and scaffold anchoring in the final stages of genome assembly. In this study, two Lotus sp. RIL populations, Lotus japonicus MG-20 × Gifu and Gifu × L. burttii, were characterized by Illumina re-sequencing. Genotyping of 187 MG-20 × Gifu RILs at 87,140 marker positions and 96 Gifu × L. burttii RILs at 357,973 marker positions allowed us to accurately identify 1,929 recombination breakpoints in the MG-20 × Gifu RILs and 1,044 breakpoints in the Gifu × L. burttii population. The resulting high-density genetic maps now facilitate high-accuracy QTL mapping, identification of reference genome mis-assemblies, and characterization of structural variants.
巻・号 23(5)
ページ 487-494
公開日 2016-10-1
DOI 10.1093/dnares/dsw033
PII dsw033
PMID 27374610
PMC PMC5066174
IF 4.009
引用数 2
リソース情報
ミヤコグサ・ダイズ LjMG RILines