RRC ID 46998
著者 Dong Q, Yin FX, Gao F, Shen Y, Zhang F, Li Y, He H, Gonzalez M, Yang J, Zhang S, Su M, Chen YH, Li F.
タイトル Ccp1 Homodimer Mediates Chromatin Integrity by Antagonizing CENP-A Loading.
ジャーナル Mol Cell
Abstract CENP-A is a centromere-specific histone 3 variant essential for centromere specification. CENP-A partially replaces canonical histone H3 at the centromeres. How the particular CENP-A/H3 ratio at centromeres is precisely maintained is unknown. It also remains unclear how CENP-A is excluded from non-centromeric chromatin. Here, we identify Ccp1, an uncharacterized NAP family protein in fission yeast that antagonizes CENP-A loading at both centromeric and non-centromeric regions. Like the CENP-A loading factor HJURP, Ccp1 interacts with CENP-A and is recruited to centromeres at the end of mitosis in a Mis16-dependent manner. These data indicate that factors with opposing CENP-A loading activities are recruited to centromeres. Furthermore, Ccp1 also cooperates with H2A.Z to evict CENP-A assembled in euchromatin. Structural analyses indicate that Ccp1 forms a homodimer that is required for its anti-CENP-A loading activity. Our study establishes mechanisms for maintenance of CENP-A homeostasis at centromeres and the prevention of ectopic assembly of centromeres.
巻・号 64(1)
ページ 79-91
公開日 2016-10-6
DOI 10.1016/j.molcel.2016.08.022
PMID 27666591
PMC PMC5055466
MeSH Binding Sites Carboxypeptidases / chemistry Carboxypeptidases / genetics* Carboxypeptidases / metabolism Carrier Proteins / chemistry Carrier Proteins / genetics* Carrier Proteins / metabolism Centromere / chemistry Centromere / metabolism Centromere / ultrastructure Chromatin Assembly and Disassembly Chromosomal Proteins, Non-Histone / chemistry Chromosomal Proteins, Non-Histone / genetics* Chromosomal Proteins, Non-Histone / metabolism Euchromatin / chemistry* Euchromatin / metabolism Euchromatin / ultrastructure Gene Expression Regulation, Fungal* Histones / chemistry Histones / genetics Histones / metabolism Mitosis Protein Binding Protein Conformation, alpha-Helical Protein Conformation, beta-Strand Protein Interaction Domains and Motifs Protein Multimerization Schizosaccharomyces / genetics* Schizosaccharomyces / metabolism Schizosaccharomyces / ultrastructure Schizosaccharomyces pombe Proteins / chemistry Schizosaccharomyces pombe Proteins / genetics* Schizosaccharomyces pombe Proteins / metabolism Signal Transduction
IF 15.584
引用数 8
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
酵母 S. pombe strains