RRC ID 11456
著者 Sato T, Kurihara H, Akimoto N, Noguchi N, Sasatsu M, Ito A.
タイトル Augmentation of gene expression and production of promatrix metalloproteinase 2 by Propionibacterium acnes-derived factors in hamster sebocytes and dermal fibroblasts: a possible mechanism for acne scarring.
ジャーナル Biol Pharm Bull
Abstract Aberrant extracellular matrix (ECM) remodeling in sebaceous glands and pilosebaceous units in the skin is associated with scar formation under acne conditions. To investigate the involvement of Propionibacterium acnes (P. acnes), a Gram-positive anaerobic microbial species, in ECM remodeling in sebaceous glands and pilosebaceous units, we examined the effects of P. acnes culture media, formalin-fixed P. acnes, and peptidoglycan (PGN) from Gram-positive bacteria walls on the production of promatrix metalloproteinase 2 (proMMP-2)/progelatinase A in hamster sebocytes and dermal fibroblasts. When hamster sebocytes (1.8×10(5) cells) and dermal fibroblasts (1×10(5) cells) were treated with P. acnes culture media and formalin-fixed P. acnes (corresponding to 1×10(6) and 1×10(7) bacterial cells), the production of proMMP-2 was augmented. In addition, PGN (5-50 µg/ml) dose-dependently augmented the production of proMMP-2 in both cells. Furthermore, the PGN (50 µg/ml)-augmented proMMP-2 production was resulted from an increase of its transcript. In contrast, there were no changes in cell proliferative activity in either the P. acnes or PGN-treated sebocytes and dermal fibroblasts, indicating that the augmented proMMP-2 production was not due to an increase in cell numbers. Therefore, these results provide novel evidence that PGN transcriptionally up-regulates the production of proMMP-2 in hamster sebocytes and dermal fibroblasts. Given an increase in the quantity of Gram-positive bacteria, including P. acnes in acne lesions, the aberrant ECM degradation may progress in sebaceous glands and pilosebaceous units, which is associated with acne scar formation.
巻・号 34(2)
ページ 295-9
公開日 2011-1-1
DOI 10.1248/bpb.34.295
PII JST.JSTAGE/bpb/34.295
PMID 21415544
MeSH Acne Vulgaris / metabolism* Acne Vulgaris / microbiology Acne Vulgaris / pathology Animals Cell Wall / chemistry Cicatrix / etiology* Cricetinae Enzyme Precursors / biosynthesis Extracellular Matrix / metabolism Extracellular Matrix / microbiology Fibroblasts / metabolism Fibroblasts / microbiology Fibroblasts / pathology Gelatinases / biosynthesis Gene Expression / drug effects* Gram-Positive Bacterial Infections / metabolism Gram-Positive Bacterial Infections / microbiology Gram-Positive Bacterial Infections / pathology Male Matrix Metalloproteinase 2 / biosynthesis* Matrix Metalloproteinase 2 / genetics Peptidoglycan / pharmacology* Propionibacterium acnes / chemistry* Sebaceous Glands / metabolism Sebaceous Glands / microbiology Sebaceous Glands / pathology Skin / metabolism* Skin / microbiology Skin / pathology Transcriptional Activation Up-Regulation
IF 1.863
引用数 14
WOS 分野 PHARMACOLOGY & PHARMACY
リソース情報
一般微生物 JCM 6473