RRC ID 33134
著者 Zhao XM, Liu C, Jiang LJ, Li QY, Zhou MT, Cheng TC, Mita K, Xia QY.
タイトル A juvenile hormone transcription factor Bmdimm-fibroin H chain pathway is involved in the synthesis of silk protein in silkworm, Bombyx mori.
ジャーナル J Biol Chem
Abstract The genes responsible for silk biosynthesis are switched on and off at particular times in the silk glands of Bombyx mori. This switch appears to be under the control of endogenous and exogenous hormones. However, the molecular mechanisms by which silk protein synthesis is regulated by the juvenile hormone (JH) are largely unknown. Here, we report a basic helix-loop-helix transcription factor, Bmdimm, its silk gland-specific expression, and its direct involvement in the regulation of fibroin H-chain (fib-H) by binding to an E-box (CAAATG) element of the fib-H gene promoter. Far-Western blots, enzyme-linked immunosorbent assays, and co-immunoprecipitation assays revealed that Bmdimm protein interacted with another basic helix-loop-helix transcription factor, Bmsage. Immunostaining revealed that Bmdimm and Bmsage proteins are co-localized in nuclei. Bmdimm expression was induced in larval silk glands in vivo, in silk glands cultured in vitro, and in B. mori cell lines after treatment with a JH analog. The JH effect on Bmdimm was mediated by the JH-Met-Kr-h1 signaling pathway, and Bmdimm expression did not respond to JH by RNA interference with double-stranded BmKr-h1 RNA. These data suggest that the JH regulatory pathway, the transcription factor Bmdimm, and the targeted fib-H gene contribute to the synthesis of fibroin H-chain protein in B. mori.
巻・号 290(2)
ページ 972-86
公開日 2015-1-9
DOI 10.1074/jbc.M114.606921
PII S0021-9258(20)57875-7
PMID 25371208
PMC PMC4294524
MeSH Animals Basic Helix-Loop-Helix Transcription Factors / biosynthesis Basic Helix-Loop-Helix Transcription Factors / genetics* Bombyx / genetics Fibroins / genetics* Fibroins / metabolism Insect Proteins / biosynthesis Insect Proteins / genetics* Juvenile Hormones / genetics* Juvenile Hormones / metabolism Larva Promoter Regions, Genetic / genetics Sericins / biosynthesis Sericins / genetics Silk / biosynthesis*
IF 4.238
引用数 19
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
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