RRC ID 49561
著者 Nihongaki Y, Furuhata Y, Otabe T, Hasegawa S, Yoshimoto K, Sato M.
タイトル CRISPR-Cas9-based photoactivatable transcription systems to induce neuronal differentiation.
ジャーナル Nat Methods
Abstract Our improved CRISPR-Cas9-based photoactivatable transcription systems, CPTS2.0 and Split-CPTS2.0, enable high blue-light-inducible activation of endogenous target genes in various human cell lines. We achieved reversible activation of target genes with CPTS2.0 and induced neuronal differentiation in induced pluripotent stem cells (iPSCs) by upregulating NEUROD1 with Split-CPTS2.0.
巻・号 14(10)
ページ 963-966
公開日 2017-10-1
DOI 10.1038/nmeth.4430
PII nmeth.4430
PMID 28892089
MeSH CRISPR-Cas Systems* Cell Differentiation Cells, Cultured DNA, Complementary Fibroblasts Gene Expression Regulation / physiology Humans Light Luminescent Measurements Neurons / physiology* Optogenetics / methods* Transcription, Genetic
IF 30.822
引用数 48
リソース情報
遺伝子材料 pCAGGS (RDB08938).
ヒト・動物細胞 HFSKF-II(RCB0698) 201B7(HPS0063) 454E2(HPS0077)