RRC ID 50850
著者 Ma J, Zhang L, Wang G, Zhang S, Zhang X, Wang Y, Shi C, Si L, Zhao H, Liu F, Zhang B, Huang X.
タイトル Rhodococcus gannanensis sp. nov., a novel endophytic actinobacterium isolated from root of sunflower (Helianthus annuus L.).
ジャーナル Antonie Van Leeuwenhoek
Abstract A novel Gram-positive, aerobic, non-motile strain, designated strain M1T, was isolated from sunflower root (Helianthus annuus L.) and characterised using a polyphasic taxonomic approach. The morphological and chemotaxonomic properties of the isolate were typical of those of members of the genus Rhodococcus. Phylogenetic analyses based on 16S rRNA gene sequence showed that strain M1T belongs to the genus Rhodococcus and clustered with Rhodococcus canchipurensis MBRL 353T (99.1%, sequence similarity) and Rhodococcus pedocola UC12T (98.7%). However, the DNA-DNA hybridizations between strain M1T and R. canchipurensis MBRL 353T and R. pedocola UC12T were found to be 52.8 ± 0.7 and 41.8 ± 0.2%, respectively. The optimal growth temperature and pH for strain M1T was found to be at 28 °C and at pH 7.0. The peptidoglycan was found to contain meso-diaminopimelic acid; galactose, glucose and arabinose were detected as diagnostic sugars. The main polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside; MK-8(H2) was found to be the dominant menaquinone. The major fatty acids were identified as C16:0, 10-methyl C18:0 and C18:1 ω9c. Mycolic acids were found to be present. The G+C content of the genomic DNA was determined to be 69.5 mol%. Based on a comparative analysis of phenotypic and genotypic characteristics, in combination with DNA-DNA hybridization results, the isolate is concluded to represent a novel species of the genus Rhodococcus, for which the name Rhodococcus gannanensis sp. nov. is proposed. The type strain is M1T (=CGMCC 1.15992T = DSM 104003T).
巻・号 110(9)
ページ 1113-1120
公開日 2017-9-1
DOI 10.1007/s10482-017-0884-9
PII 10.1007/s10482-017-0884-9
PMID 28488064
MeSH Base Composition Cell Wall / chemistry Endophytes / chemistry Endophytes / classification Endophytes / genetics Helianthus / microbiology* Nucleic Acid Hybridization Phylogeny* Plant Roots / microbiology RNA, Ribosomal, 16S / genetics Rhodococcus / chemistry Rhodococcus / classification* Rhodococcus / genetics* Species Specificity
IF 2.674
引用数 2
リソース情報
一般微生物 JCM 17578