RRC ID 52696
著者 Tsuchida A, Senda M, Ito A, Saito S, Kiso M, Ando T, Harduin-Lepers A, Matsuda A, Furukawa K, Furukawa K.
タイトル Roles of GalNAc-disialyl Lactotetraosyl Antigens in Renal Cancer Cells.
ジャーナル Sci Rep
Abstract GalNAc-disialyl Lc4 (GalNAc-DSLc4) was reported as a novel antigen that associated with malignant features of renal cell cancers (RCCs). To clarify roles of GalNAc-DSLc4 in malignant properties of RCCs, we identified B4GalNAc-T2 as a responsible gene for the synthesis of GalNAc-DSLc4, and prepared stable transfectants of GalNAc-T2 cDNA using VMRC-RCW cells, resulting in the establishment of high expressants of GalNAc-DSLc4. They showed increased proliferation and invasion, and specific adhesion to laminin. In the transfectants, PI3K/Akt signals were highly activated by serum stimulation or adhesion to laminin. GalNAc-DSLc4 was co-localized in lipid rafts with integrin β1 and caveolin-1 in both immunoblotting of fractionated detergent extracts and immunocytostaining, particularly when stimulated with serum. Masking of GalNAc-DSLc4 with antibodies as well as PI3K inhibitor suppressed malignant properties of the transfectants. These results suggested that GalNAc-DSLc4 is involved in malignant properties of RCCs by forming a molecular complex with integrins in lipid rafts.
巻・号 8(1)
ページ 7017
公開日 2018-5-4
DOI 10.1038/s41598-018-25521-6
PII 10.1038/s41598-018-25521-6
PMID 29728594
PMC PMC5935701
MeSH Antigens, Neoplasm / immunology* Carcinoma, Renal Cell / immunology* Carcinoma, Renal Cell / metabolism Cell Adhesion Cell Line, Tumor Cell Movement Cell Proliferation Glycolipids Humans Integrins / metabolism Kidney Neoplasms / immunology* Kidney Neoplasms / metabolism Phosphorylation Protein Transport Proto-Oncogene Proteins c-akt / metabolism Signal Transduction
IF 3.998
引用数 1
リソース情報
ヒト・動物細胞 OS-RC-2(RCB0735) RCC10RGB(RCB1151) TUHR4TKB(RCB1198)