RRC ID 15837
著者 Yamazaki S, Yoshinari S, Kita K, Watanabe Y, Kawarabayasi Y.
タイトル Identification of an entire set of tRNA molecules and characterization of cleavage sites of the intron-containing tRNA precursors in acidothermophilic crenarchaeon Sulfolobus tokodaii strain7.
ジャーナル Gene
Abstract The acidothermophilic crenarchaeon, Sulfolobus tokodaii strain7, was isolated from a hot spring in Beppu, Kyushu, Japan. Whole genomic data of this microorganism indicated that among 46 putative tRNA genes identified, 24 were interrupted tRNA genes containing an intron. A sequence comparison between the cDNA sequences for unspliced and spliced tRNAs indicated that all predicted tRNAs were expressed and all intron portions were spliced in this microorganism. However, the actual cleavage site in the splicing process was not determined for 13 interrupted tRNAs because of the presence of the same nucleotides at both 5' and 3' border regions of each intron. The cleavage sites for all the introns, which were determined by an in vitro cleavage experiment with recombinant splicing endonuclease as well as cDNA sequencing of the spliced tRNAs, indicated that non-canonical BHB structure motifs were also recognized and processed by the splicing machinery in this organism. This is the first report to empirically determine the actual cleavage and splice sites of introns in the whole set of archaeal tRNA genes, and reassigns the exon-intron borders with a novel and more plausible non-canonical BHB structure.
巻・号 489(2)
ページ 103-10
公開日 2011-12-10
DOI 10.1016/j.gene.2011.08.003
PII S0378-1119(11)00395-7
PMID 21872648
MeSH Anticodon Base Sequence Genome, Archaeal Hot Springs / microbiology Nucleic Acid Conformation RNA Cleavage* RNA Precursors / chemistry RNA Precursors / genetics* RNA Splice Sites / genetics* RNA Splicing RNA, Archaeal / chemistry RNA, Archaeal / genetics RNA, Transfer / chemistry RNA, Transfer / genetics* Sequence Alignment Sulfolobus / genetics*
IF 2.984
引用数 2
WOS 分野 GENETICS & HEREDITY
リソース情報
一般微生物 JCM 10545