RRC ID |
48162
|
著者 |
Abagana AY, Sun P, Liu C, Cao T, Zheng W, Zhao S, Xiang W, Wang X.
|
タイトル |
Actinomadura gamaensis sp. nov., a novel actinomycete isolated from soil in Gama, Chad.
|
ジャーナル |
Antonie Van Leeuwenhoek
|
Abstract |
A novel single spore-producing actinomycete, designated strain NEAU-Gz5(T), was isolated from a soil sample from Gama, Chad. A polyphasic taxonomic study was carried out to establish the status of this strain. The diamino acid present in the cell wall is meso-diaminopimelic acid. Glucose, mannose and madurose occur in whole cell hydrolysates. The polar lipids were found to consist of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannoside and an unidentified glycolipid. The predominant menaquinones were identified as MK-9(H8) and MK-9(H6). The predominant cellular fatty acids were found to be C16:0, iso-C15:0, iso-C16:0 and C18:0 10-methyl. Phylogenetic analysis based on the 16S rRNA gene showed that strain NEAU-Gz5(T) belongs to the genus Actinomadura and is closely related to Actinomadura oligospora JCM 10648(T) (ATCC 43269(T); 98.3 % similarity). However, the low level of DNA-DNA relatedness and some different phenotypic characteristics allowed the strain to be distinguished from its close relatives. Therefore, it is concluded that strain NEAU-Gz5(T) represents a novel species of the genus of Actinomadura, for which the name Actinomadura gamaensis sp. nov. is proposed. The type strain is NEAU-Gz5(T) (= CGMCC 4.7301(T) = DSM 100815(T)).
|
巻・号 |
109(6)
|
ページ |
833-9
|
公開日 |
2016-6-1
|
DOI |
10.1007/s10482-016-0683-8
|
PII |
10.1007/s10482-016-0683-8
|
PMID |
27010208
|
MeSH |
Actinomycetales / classification*
Actinomycetales / genetics
Actinomycetales / isolation & purification*
Actinomycetales / metabolism
Bacterial Typing Techniques
Cell Wall / metabolism
Chad
DNA, Bacterial / genetics
DNA, Ribosomal / genetics
Diaminopimelic Acid / metabolism
Fatty Acids / analysis
Nucleic Acid Hybridization
Peptidoglycan / metabolism
Phospholipids / metabolism
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Soil / chemistry
Soil Microbiology*
Spores, Bacterial
Vitamin K 2 / metabolism
|
IF |
2.674
|
引用数 |
4
|
WOS 分野
|
MICROBIOLOGY
|
リソース情報 |
一般微生物 |
JCM 10648 |