RRC ID 50946
著者 Tawthep S, Fukiya S, Lee JY, Hagio M, Ogura Y, Hayashi T, Yokota A.
タイトル Isolation of six novel 7-oxo- or urso-type secondary bile acid-producing bacteria from rat cecal contents.
ジャーナル J Biosci Bioeng
Abstract Understanding the dynamics of secondary bile acid (SBA) formation in the gut by SBA-producing bacteria is important for host health, as SBAs have been shown to affect host pathophysiology and gut microbiota composition. However, our knowledge of SBA producers is limited in light of the diversity of gut microbes. Here, we isolated six novel SBA-producing bacteria from rat cecal contents, all of which were members of known species of gut microbes. Anaerostipes caccae D10, Bacteroides nordii C5, Clostridioides difficile D7, and Clostridium cadaveris G11 were capable of oxidizing cholic acid and chenodeoxycholic acid into 7-oxo-derivatives with varying yields. B. nordii C5 and its type strain JCM 12987T had the highest molar yield, ∼90%. Clostridium disporicum F4 and Clostridium subterminale C4 epimerized cholic acid into ursocholic acid with yields of ∼85%; the corresponding type strains lacked epimerization activity. Furthermore, although not novel as an SBA producer, Clostridium scindens G10 that produced deoxycholic acid from cholic acid was isolated for the first time from rodents. These findings will contribute to elucidation of SBA formation in the gut.
巻・号 124(5)
ページ 514-522
公開日 2017-11-1
DOI 10.1016/j.jbiosc.2017.06.002
PII S1389-1723(17)30334-1
PMID 28751127
MeSH Animals Bacteria / isolation & purification* Bacteria / metabolism* Bacteroides / isolation & purification Bacteroides / metabolism Bile Acids and Salts / biosynthesis* Cecum / microbiology* Chenodeoxycholic Acid / metabolism Cholic Acid / metabolism Cholic Acids / metabolism Clostridium / isolation & purification Clostridium / metabolism Deoxycholic Acid / metabolism Male Rats
IF 2.366
引用数 6
リソース情報
一般微生物 JCM 12987 JCM 13470 JCM 1296 JCM 1392 JCM 6567 JCM 1417 JCM 1401