RRC ID 52628
著者 Yajima Y, Lee CN, Yamada M, Utoh R, Seki M.
タイトル Development of a perfusable 3D liver cell cultivation system via bundling-up assembly of cell-laden microfibers.
ジャーナル J Biosci Bioeng
Abstract Although the reconstruction of functional 3D liver tissue models in vitro presents numerous challenges, it is in great demand for drug development, regenerative medicine, and physiological studies. Here we propose a new approach to perform perfusion cultivation of liver cells by assembling cell-laden hydrogel microfibers. HepG2 cells were densely packed into the core of sandwich-type anisotropic microfibers, which were produced using microfluidic devices. The obtained microfibers were bundled up and packed into a perfusion chamber, and perfusion cultivation was performed. We evaluated cell viability and functions, and also monitored the oxygen consumption. Furthermore, fibers covered with vascular endothelial cells were united during the perfusion culture, to form vascular network-like conduits between fibers. The presented technique can structurally mimic the hepatic lobule in vivo and could prove to be a useful model for various biomedical research applications.
巻・号 126(1)
ページ 111-118
公開日 2018-7-1
DOI 10.1016/j.jbiosc.2018.01.022
PII S1389-1723(17)31108-8
PMID 29502942
MeSH Animals Cattle Cell Culture Techniques* / instrumentation Cell Culture Techniques* / methods Cells, Cultured Coculture Techniques Endothelial Cells / cytology Hep G2 Cells Hepatocytes / cytology* Humans Hydrogel, Polyethylene Glycol Dimethacrylate / chemistry Lab-On-A-Chip Devices Liver / cytology* Microtechnology / instrumentation Microvessels / cytology Perfusion Regenerative Medicine / instrumentation Regenerative Medicine / methods Tissue Engineering / instrumentation* Tissue Engineering / methods* Tissue Scaffolds
IF 2.366
引用数 13
リソース情報
ヒト・動物細胞 Hep G2(RCB1886)