RRC ID 45879
著者 Wang X, Liu J, Zhu Z, Ou G.
タイトル The heparan sulfate-modifying enzyme glucuronyl C5-epimerase HSE-5 controls Caenorhabditis elegans Q neuroblast polarization during migration.
ジャーナル Dev Biol
Abstract Directional cell migration is fundamental for neural development, and extracellular factors are pivotal for this process. Heparan sulfate proteoglycans (HSPGs) that carry long chains of differentially modified sugar residues contribute to extracellular matrix; however, the functions of HSPG in guiding cell migration remain elusive. Here, we used the Caenorhabditis elegans mutant pool from the Million Mutation Project and isolated a mutant allele of the heparan sulfate-modifying enzyme glucuronyl C5-epimerase HSE-5. Loss-of-function of this enzyme resulted in defective Q neuroblast migration. We showed that hse-5 controlled Q cell migration in a cell non-autonomous manner. By performing live cell imaging in hse-5 mutant animals, we found that hse-5 controlled initial polarization during Q neuroblast migration. Furthermore, our genetic epistasis analysis demonstrated that lon-2 might act downstream of hse-5. Finally, rescue of the hse-5 mutant phenotype by expression of human and mouse hse-5 homologs suggested a conserved function for this gene in neural development. Taken together, our results indicated that proper HSPG modification in the extracellular matrix by HSE-5 is essential for neuroblast polarity during migration.
巻・号 399(2)
ページ 306-14
公開日 2015-3-15
DOI 10.1016/j.ydbio.2015.01.007
PII S0012-1606(15)00015-9
PMID 25614236
MeSH Animals Caenorhabditis elegans / enzymology Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Carbohydrate Epimerases / genetics Carbohydrate Epimerases / metabolism* Cell Movement / genetics Cell Movement / physiology* Cell Polarity / genetics Cell Polarity / physiology* Epistasis, Genetic / physiology Humans Mice Microscopy, Confocal Neural Stem Cells / physiology* Racemases and Epimerases / metabolism* Species Specificity
IF 2.896
引用数 6
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
線虫 tm472