RRC ID 34467
著者 Kimura T, Ishikawa C, Osorio-Lozada A, Robins KT, Hibi M, Ogawa J.
タイトル Production of a pharmaceutical intermediate via biohydroxylation using whole cells of Rhodococcus rubropertinctus N82.
ジャーナル Biosci Biotechnol Biochem
Abstract Rhodococcus rubropertinctus N82 possesses unique regiospecific hydroxylation activity in biotransformation of compounds. In this study, the ability of whole cells of the strain R. rubropertinctus N82 in biotransformation was studied. The hydroxylation activity resulted in transforming 6,7-dihydro-4H-thieno[3,2-c]-pyridine-5-carboxylic acid tert-butyl ester (LS1) into 2-hydroxy-6,7-dihydro-4H-thieno[3,2-c]-pyridine-5-carboxylic acid tert-butyl ester (LP1), a pharmaceutical intermediate. By optimizing conditions for the hydroxylating biotransformation using whole cells of R. rubropertinctus N82 as biocatalyst, 3.3 mM LP1 was successfully produced from 4 mM LS1 with a molar yield of 83%. Thus, effective method was newly developed to produce LP1, which is a synthetic intermediate of a platelet inhibitor active pharmaceutical ingredient drug, prasugrel.
巻・号 78(10)
ページ 1772-6
公開日 2014-1-1
DOI 10.1080/09168451.2014.925781
PMID 25273144
MeSH Biotransformation Culture Techniques Hydroxylation Pharmaceutical Preparations / metabolism* Rhodococcus / cytology* Rhodococcus / growth & development Rhodococcus / metabolism*
IF 1.516
引用数 3
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY
リソース情報
一般微生物 JCM 3204