RRC ID 37520
著者 Nakagami G, Minematsu T, Morohoshi T, Yamane T, Kanazawa T, Huang L, Asada M, Nagase T, Ikeda S, Ikeda T, Sanada H.
タイトル Pseudomonas aeruginosa quorum-sensing signaling molecule N-3-oxododecanoyl homoserine lactone induces matrix metalloproteinase 9 expression via the AP1 pathway in rat fibroblasts.
ジャーナル Biosci Biotechnol Biochem
Abstract Quorum sensing is a cell-to-cell communication mechanism, which is responsible for regulating a number of bacterial virulence factors and biofilm maturation and therefore plays an important role for establishing wound infection. Quorum-sensing signals may induce inflammation and predispose wounds to infection by Pseudomonas aeruginosa; however, the interaction has not been well investigated. We examined the effects of the P. aeruginosa las quorum-sensing signal, N-3-oxo-dodecanoyl homoserine lactone (3OC12-HSL), on matrix metalloproteinase (MMP) 9 expression in Rat-1 fibroblasts. 3OC12-HSL upregulated the expression of the MMP9 gene bearing an activator protein-1 (AP-1) binding site in the promoter region. We further investigated the mechanism underlying this effect. c-Fos gene expression increased rapidly after exposure to 3OC12-HSL, and nuclear translocation of c-Fos protein was observed; both effects were reduced by pretreatment with an AP-1 inhibitor. These results suggest that 3OC12-HSL can alter MMP9 gene expression in fibroblasts via the AP-1 signaling pathway.
巻・号 79(10)
ページ 1719-24
公開日 2015-1-1
DOI 10.1080/09168451.2015.1056509
PMID 26096293
MeSH 4-Butyrolactone / analogs & derivatives* 4-Butyrolactone / pharmacology Abietanes / pharmacology Animals Cell Line Cell Nucleus / drug effects Cell Nucleus / metabolism Fibroblasts / cytology Fibroblasts / drug effects* Fibroblasts / metabolism Gene Expression Regulation / drug effects* Homoserine / analogs & derivatives* Homoserine / pharmacology Matrix Metalloproteinase 9 / genetics* Matrix Metalloproteinase 9 / metabolism Promoter Regions, Genetic Protein Transport Proto-Oncogene Proteins c-fos / genetics Proto-Oncogene Proteins c-fos / metabolism Pseudomonas aeruginosa / chemistry* Pseudomonas aeruginosa / metabolism Quorum Sensing Rats Signal Transduction Transcription Factor AP-1 / antagonists & inhibitors Transcription Factor AP-1 / genetics* Transcription Factor AP-1 / metabolism
IF 1.516
引用数 4
WOS 分野 FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY CHEMISTRY, APPLIED BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 Rat-1(RCB1830)